Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

demonstrating growth in a nitrogen free medium
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
measuring15N2 by mass spectrometer
All of these

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Microbiology of Soils
The groups of symbiotic bacteria, which have the ability to fix nitrogen

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these bacteria are from the genus, Rhizobium
grow together for a mutual benefit is called symbiosis and so these bacteria are called symbiotic nitrogen-fixing bacteria
derive their food and minerals from the legume, and in turn they supply the legume with some or all of its nitrogen

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