Microbiology of Soils
A heterocyst is

the progenitor of cyanobacterial vegetative cells
a type of spore
a terminally differentiated cell that fixes nitrogen
a cell that carries out oxygenic photosynthesis

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Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

measuring15N2 by mass spectrometer
All of these
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
demonstrating growth in a nitrogen free medium

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