Microbiology of Soils
The groups of symbiotic bacteria, which have the ability to fix nitrogen

All of these
derive their food and minerals from the legume, and in turn they supply the legume with some or all of its nitrogen
grow together for a mutual benefit is called symbiosis and so these bacteria are called symbiotic nitrogen-fixing bacteria
these bacteria are from the genus, Rhizobium

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Microbiology of Soils
Denitrification is

reduction of nitrate (NO3-) to nitrogen gas
reduction of nitrate to organic nitrogen compounds
both (a) and (b)
changing of atmospheric nitrogen (N2) to nitrogen compounds

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Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

measuring15N2 by mass spectrometer
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
demonstrating growth in a nitrogen free medium
All of these

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