Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
measuring15N2 by mass spectrometer
All of these
demonstrating growth in a nitrogen free medium

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Microbiology of Soils
Nitrogen oxidation (nitrification)refers to the

None of these
sulfur is oxidized to the sulfate form through Thiobacillus bacteria
conversion of ammonium ions into nitrates through the activities of certain bacteria.
changing of atmospheric nitrogen (N2) to nitrogen compounds

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Microbiology of Soils
The groups of symbiotic bacteria, which have the ability to fix nitrogen

grow together for a mutual benefit is called symbiosis and so these bacteria are called symbiotic nitrogen-fixing bacteria
All of these
these bacteria are from the genus, Rhizobium
derive their food and minerals from the legume, and in turn they supply the legume with some or all of its nitrogen

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