Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

measuring15N2 by mass spectrometer
demonstrating growth in a nitrogen free medium
All of these
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen

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Microbiology of Soils
The groups of symbiotic bacteria, which have the ability to fix nitrogen

these bacteria are from the genus, Rhizobium
All of these
derive their food and minerals from the legume, and in turn they supply the legume with some or all of its nitrogen
grow together for a mutual benefit is called symbiosis and so these bacteria are called symbiotic nitrogen-fixing bacteria

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