Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

measuring15N2 by mass spectrometer
All of these
demonstrating growth in a nitrogen free medium
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen

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Microbiology of Soils
Denitrification is

changing of atmospheric nitrogen (N2) to nitrogen compounds
reduction of nitrate to organic nitrogen compounds
both (a) and (b)
reduction of nitrate (NO3-) to nitrogen gas

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