Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen
measuring15N2 by mass spectrometer
All of these
demonstrating growth in a nitrogen free medium

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Microbiology of Soils
Nitrogen fixation

fix the free nitrogen (N2) by which it should not be usable by plants
changes the free nitrogen (N2) to a form usable by plants
especially changes nitrogen compounds, mostly amines such as NH2
both (a) and (b)

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