Glycolysis
During glycolysis, the major energy generating step involves

pyruvate kinase
Phosphofructokinase
glyceraldehyde-3 -dehydrogenase
phosphoglycerate kinase

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Glycolysis
A kinase is an enzyme that

uses ATP to add a phosphate group to the substrate
uses NADH to change the oxidation state of the substrate
removes water from a double bond
removes phosphate groups of substrates

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