Glycolysis
During glycolysis, the major energy generating step involves

pyruvate kinase
glyceraldehyde-3 -dehydrogenase
Phosphofructokinase
phosphoglycerate kinase

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Glycolysis
Which of the following is not true of glycolysis?

ADP is phosphorylated to ATP via substrate level phosphorylation
The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose
The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters
The pathway does not require oxygen

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

None of these
the techniques for isolating the phosphate donor are not refined enough
no such phosphate donor exists
the high-energy phosphate donor is very short-lived and difficult to isolate

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