Glycolysis
During glycolysis, the major energy generating step involves

phosphoglycerate kinase
Phosphofructokinase
glyceraldehyde-3 -dehydrogenase
pyruvate kinase

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

the techniques for isolating the phosphate donor are not refined enough
None of these
the high-energy phosphate donor is very short-lived and difficult to isolate
no such phosphate donor exists

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Glycolysis
Why does the glycolytic pathway continue in the direction of glucose catabolism?

High levels of ATP keep the pathway going in a forward direction
Glycolysis occurs in either direction
The enzymes of glycolysis only function in one direction
There are essentially three irreversible reactions that act as the driving force for the pathway

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