Glycolysis
Why does the glycolytic pathway continue in the direction of glucose catabolism?

Glycolysis occurs in either direction
The enzymes of glycolysis only function in one direction
There are essentially three irreversible reactions that act as the driving force for the pathway
High levels of ATP keep the pathway going in a forward direction

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

the high-energy phosphate donor is very short-lived and difficult to isolate
no such phosphate donor exists
None of these
the techniques for isolating the phosphate donor are not refined enough

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Glycolysis
A kinase is an enzyme that

uses NADH to change the oxidation state of the substrate
removes phosphate groups of substrates
uses ATP to add a phosphate group to the substrate
removes water from a double bond

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