Glycolysis
The enzymes of glycolysis in a eukaryotic cell are located in the

cytosol
intermembrane space
mitochondrial matrix
plasma membrane

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Glycolysis
A kinase is an enzyme that

uses NADH to change the oxidation state of the substrate
uses ATP to add a phosphate group to the substrate
removes phosphate groups of substrates
removes water from a double bond

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

the techniques for isolating the phosphate donor are not refined enough
None of these
no such phosphate donor exists
the high-energy phosphate donor is very short-lived and difficult to isolate

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