Glycolysis
Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

Covalent modification of the enzyme
Allosteric control of the enzyme activity
Genetic control of the enzyme concentration
Diffusional coupling between adjacent active sites

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

None of these
the high-energy phosphate donor is very short-lived and difficult to isolate
the techniques for isolating the phosphate donor are not refined enough
no such phosphate donor exists

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