Glycolysis
Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

Genetic control of the enzyme concentration
Covalent modification of the enzyme
Allosteric control of the enzyme activity
Diffusional coupling between adjacent active sites

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Glycolysis
A kinase is an enzyme that

removes water from a double bond
uses NADH to change the oxidation state of the substrate
uses ATP to add a phosphate group to the substrate
removes phosphate groups of substrates

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

None of these
the techniques for isolating the phosphate donor are not refined enough
the high-energy phosphate donor is very short-lived and difficult to isolate
no such phosphate donor exists

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Glycolysis
Which of the following is not true of glycolysis?

The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters
The pathway does not require oxygen
ADP is phosphorylated to ATP via substrate level phosphorylation
The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose

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