Glycolysis
Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

Diffusional coupling between adjacent active sites
Genetic control of the enzyme concentration
Covalent modification of the enzyme
Allosteric control of the enzyme activity

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

the techniques for isolating the phosphate donor are not refined enough
no such phosphate donor exists
None of these
the high-energy phosphate donor is very short-lived and difficult to isolate

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