Glycolysis
Fructose-2,6-bisphosphate

activates phosphofructokinase
inhibits fructose-1,6-bisphosphatase
activates fructose-1,6-bisphosphatase
both (b) and (c)

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Glycolysis
A kinase is an enzyme that

uses ATP to add a phosphate group to the substrate
removes phosphate groups of substrates
removes water from a double bond
uses NADH to change the oxidation state of the substrate

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

no such phosphate donor exists
the techniques for isolating the phosphate donor are not refined enough
None of these
the high-energy phosphate donor is very short-lived and difficult to isolate

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