Industrially Useful Microbial Processes
The early assay employed for measuring penicillin activity were based upon the quantity required to prevent growth of S. aureus over

200 mm diameter zone using the cylinder plate method of assay
None of these
20 mm diameter zone using the cylinder plate method of assay
24 mm diameter zone using the cylinder plate method of assay

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Industrially Useful Microbial Processes
Why high concentration of sugars are not employed in the fermentation medium?

Calcium lactate is not produced
Calcium lactate crystallizes from the medium and slows down the process of fermentation
High sugar concentration itself crystallizes
None of these

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