DNA Sequencing, Mutation and Repair
A mutagen is defined as

a chemical or physical agent that induces mutations
an enzyme that repairs mutations
a molecule which stabilizes DNA thus prevents mutations from occurring
an inhibitor of gene modification

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DNA Sequencing, Mutation and Repair
The whole-genome shotgun sequencing approach depends primarily on

methodical sequencing a few large cloned fragments of DNA
rapidly sequencing thousands of small randomly cloned fragments
All of these
sequencing the bacterial chromosome while it is still intact

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DNA Sequencing, Mutation and Repair
The DNA sequences of rRNA genes can be used to

assess relatedness of eukaryotes but not prokaryotes
predict whether a bacteria will be gram-positive or gram-negative
obtain a tentative identification of a new microorganism
predict the pulsed field gel pattern of a microorganism

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