Physical and Chemical Agents
Which of the following methods will not sterilize?

Dry heat at 171°C for 1 to 2 hours
121°C at 15 lb/in pressure for 15 to 20 minutes
100°C boiling water for 30 minutes
Aqueous glutaraldehyde for 10 hours

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Physical and Chemical Agents
Which of the following is best used for long term storage of microbial samples when carried out properly?

Storage in a freezer at -10°C
Storage on a petri plate at room temperature
Storage in a freezer at ultra low temperatures (-70°C)
Storage in a refrigerator on an agar slant

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