Physical and Chemical Agents
Which of the following methods will not sterilize?

121°C at 15 lb/in pressure for 15 to 20 minutes
100°C boiling water for 30 minutes
Aqueous glutaraldehyde for 10 hours
Dry heat at 171°C for 1 to 2 hours

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Physical and Chemical Agents
Which of the following describes the effect of ionizing radiation on microbes?

Induces electrons and protons to jump out of the molecules of the microbe
Changes the 3-dimensional structure of proteins to a 2-dimen-sional form
None of these
Substantially decreases enzymatic activity, slowing microbial reproduction

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Physical and Chemical Agents
Which of the following is best used for long term storage of microbial samples when carried out properly?

Storage on a petri plate at room temperature
Storage in a freezer at ultra low temperatures (-70°C)
Storage in a refrigerator on an agar slant
Storage in a freezer at -10°C

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