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Protein Purification

Protein Purification
Which of the following may be added to stabilize the protein after yeast cells disruption?

AMP
Protease inhibitor
NaCl
All of these

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Protein Purification
During successful purification scheme, this may be expected that the

specific activity decreases
specific activity increases
number of proteins in the sample decreases
both (a) and (c)

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Protein Purification
The best way to determine the location of protein in the purification scheme is to measure the

mass spectroscopy of the protein
changes in the refractive index
rate of ATP synthesis
UV absorption

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Protein Purification
Affinity chromatography deals with the

protein - protein interaction
None of these
protein - carbohydrate interaction
specific binding of a protein constituents for another molecule

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Protein Purification
Proteins separation can be carried out on the basis of

solubility in salt solutions
all of these
net charge
size or mass

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Protein Purification
Gel-filtration chromatography separates on the basis of

size using porous beads packed in a column
None of these
shape using porous beads packed in a column
size and shape using porous beads packed in a column

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