Protein Purification Which of the following may be added to stabilize the protein after yeast cells disruption? AMP Protease inhibitor All of these NaCl AMP Protease inhibitor All of these NaCl ANSWER DOWNLOAD EXAMIANS APP
Protein Purification The best way to determine the location of protein in the purification scheme is to measure the rate of ATP synthesis changes in the refractive index mass spectroscopy of the protein UV absorption rate of ATP synthesis changes in the refractive index mass spectroscopy of the protein UV absorption ANSWER DOWNLOAD EXAMIANS APP
Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 1,000 units/mg 100,000 units/mg 10,000 units/mg. 1,000,000 units/mg 1,000 units/mg 100,000 units/mg 10,000 units/mg. 1,000,000 units/mg ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Proteins separation can be carried out on the basis of solubility in salt solutions all of these size or mass net charge solubility in salt solutions all of these size or mass net charge ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Gel-filtration chromatography separates on the basis of None of these size using porous beads packed in a column shape using porous beads packed in a column size and shape using porous beads packed in a column None of these size using porous beads packed in a column shape using porous beads packed in a column size and shape using porous beads packed in a column ANSWER DOWNLOAD EXAMIANS APP
Protein Purification In ion-exchange chromatography either (b) or (c) proteins are separated on the basis of their size proteins are separated on the basis of their shape proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their size proteins are separated on the basis of their shape proteins are separated on the basis of their net charge ANSWER DOWNLOAD EXAMIANS APP