Protein Purification
The best way to determine the location of protein in the purification scheme is to measure the

UV absorption
changes in the refractive index
rate of ATP synthesis
mass spectroscopy of the protein

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Protein Purification
In ion-exchange chromatography

proteins are separated on the basis of their shape
either (b) or (c)
proteins are separated on the basis of their net charge
proteins are separated on the basis of their size

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