Protein Purification
The best way to determine the location of protein in the purification scheme is to measure the

UV absorption
mass spectroscopy of the protein
rate of ATP synthesis
changes in the refractive index

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Protein Purification
In ion-exchange chromatography

proteins are separated on the basis of their shape
proteins are separated on the basis of their size
either (b) or (c)
proteins are separated on the basis of their net charge

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