Protein Purification The best way to determine the location of protein in the purification scheme is to measure the mass spectroscopy of the protein rate of ATP synthesis changes in the refractive index UV absorption mass spectroscopy of the protein rate of ATP synthesis changes in the refractive index UV absorption ANSWER DOWNLOAD EXAMIANS APP
Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 100,000 units/mg 10,000 units/mg. 1,000 units/mg 1,000,000 units/mg 100,000 units/mg 10,000 units/mg. 1,000 units/mg 1,000,000 units/mg ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Proteins separation can be carried out on the basis of solubility in salt solutions net charge size or mass all of these solubility in salt solutions net charge size or mass all of these ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Affinity chromatography deals with the protein - protein interaction specific binding of a protein constituents for another molecule None of these protein - carbohydrate interaction protein - protein interaction specific binding of a protein constituents for another molecule None of these protein - carbohydrate interaction ANSWER DOWNLOAD EXAMIANS APP
Protein Purification In ion-exchange chromatography proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their shape proteins are separated on the basis of their size proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their shape proteins are separated on the basis of their size ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Gel-filtration chromatography separates on the basis of size and shape using porous beads packed in a column size using porous beads packed in a column None of these shape using porous beads packed in a column size and shape using porous beads packed in a column size using porous beads packed in a column None of these shape using porous beads packed in a column ANSWER DOWNLOAD EXAMIANS APP