Protein Purification
The best way to determine the location of protein in the purification scheme is to measure the

mass spectroscopy of the protein
rate of ATP synthesis
changes in the refractive index
UV absorption

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Protein Purification
In ion-exchange chromatography

proteins are separated on the basis of their net charge
either (b) or (c)
proteins are separated on the basis of their shape
proteins are separated on the basis of their size

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