Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 1,000 units/mg 10,000 units/mg. 1,000,000 units/mg 100,000 units/mg 1,000 units/mg 10,000 units/mg. 1,000,000 units/mg 100,000 units/mg ANSWER DOWNLOAD EXAMIANS APP
Protein Purification In ion-exchange chromatography proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their shape proteins are separated on the basis of their size proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their shape proteins are separated on the basis of their size ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Proteins separation can be carried out on the basis of net charge solubility in salt solutions all of these size or mass net charge solubility in salt solutions all of these size or mass ANSWER DOWNLOAD EXAMIANS APP
Protein Purification During successful purification scheme, this may be expected that the specific activity increases number of proteins in the sample decreases both (a) and (c) specific activity decreases specific activity increases number of proteins in the sample decreases both (a) and (c) specific activity decreases ANSWER DOWNLOAD EXAMIANS APP
Protein Purification The best way to determine the location of protein in the purification scheme is to measure the changes in the refractive index rate of ATP synthesis UV absorption mass spectroscopy of the protein changes in the refractive index rate of ATP synthesis UV absorption mass spectroscopy of the protein ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Affinity chromatography deals with the protein - carbohydrate interaction None of these specific binding of a protein constituents for another molecule protein - protein interaction protein - carbohydrate interaction None of these specific binding of a protein constituents for another molecule protein - protein interaction ANSWER DOWNLOAD EXAMIANS APP