Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 10,000 units/mg. 100,000 units/mg 1,000 units/mg 1,000,000 units/mg 10,000 units/mg. 100,000 units/mg 1,000 units/mg 1,000,000 units/mg ANSWER DOWNLOAD EXAMIANS APP
Protein Purification The best way to determine the location of protein in the purification scheme is to measure the UV absorption rate of ATP synthesis mass spectroscopy of the protein changes in the refractive index UV absorption rate of ATP synthesis mass spectroscopy of the protein changes in the refractive index ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Gel-filtration chromatography separates on the basis of None of these size and shape using porous beads packed in a column shape using porous beads packed in a column size using porous beads packed in a column None of these size and shape using porous beads packed in a column shape using porous beads packed in a column size using porous beads packed in a column ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Which of the following may be added to stabilize the protein after yeast cells disruption? NaCl AMP Protease inhibitor All of these NaCl AMP Protease inhibitor All of these ANSWER DOWNLOAD EXAMIANS APP
Protein Purification During successful purification scheme, this may be expected that the specific activity decreases both (a) and (c) number of proteins in the sample decreases specific activity increases specific activity decreases both (a) and (c) number of proteins in the sample decreases specific activity increases ANSWER DOWNLOAD EXAMIANS APP
Protein Purification In ion-exchange chromatography proteins are separated on the basis of their size proteins are separated on the basis of their net charge proteins are separated on the basis of their shape either (b) or (c) proteins are separated on the basis of their size proteins are separated on the basis of their net charge proteins are separated on the basis of their shape either (b) or (c) ANSWER DOWNLOAD EXAMIANS APP
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