Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 1,000 units/mg 100,000 units/mg 1,000,000 units/mg 10,000 units/mg. 1,000 units/mg 100,000 units/mg 1,000,000 units/mg 10,000 units/mg. ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Proteins separation can be carried out on the basis of size or mass net charge all of these solubility in salt solutions size or mass net charge all of these solubility in salt solutions ANSWER DOWNLOAD EXAMIANS APP
Protein Purification During successful purification scheme, this may be expected that the both (a) and (c) specific activity increases number of proteins in the sample decreases specific activity decreases both (a) and (c) specific activity increases number of proteins in the sample decreases specific activity decreases ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Which of the following may be added to stabilize the protein after yeast cells disruption? NaCl Protease inhibitor AMP All of these NaCl Protease inhibitor AMP All of these ANSWER DOWNLOAD EXAMIANS APP
Protein Purification In ion-exchange chromatography proteins are separated on the basis of their shape proteins are separated on the basis of their net charge proteins are separated on the basis of their size either (b) or (c) proteins are separated on the basis of their shape proteins are separated on the basis of their net charge proteins are separated on the basis of their size either (b) or (c) ANSWER DOWNLOAD EXAMIANS APP
Protein Purification The best way to determine the location of protein in the purification scheme is to measure the mass spectroscopy of the protein changes in the refractive index rate of ATP synthesis UV absorption mass spectroscopy of the protein changes in the refractive index rate of ATP synthesis UV absorption ANSWER DOWNLOAD EXAMIANS APP
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