Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 100,000 units/mg 1,000,000 units/mg 10,000 units/mg. 1,000 units/mg 100,000 units/mg 1,000,000 units/mg 10,000 units/mg. 1,000 units/mg ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Which of the following may be added to stabilize the protein after yeast cells disruption? Protease inhibitor All of these NaCl AMP Protease inhibitor All of these NaCl AMP ANSWER DOWNLOAD EXAMIANS APP
Protein Purification The best way to determine the location of protein in the purification scheme is to measure the rate of ATP synthesis changes in the refractive index mass spectroscopy of the protein UV absorption rate of ATP synthesis changes in the refractive index mass spectroscopy of the protein UV absorption ANSWER DOWNLOAD EXAMIANS APP
Protein Purification During successful purification scheme, this may be expected that the number of proteins in the sample decreases specific activity decreases specific activity increases both (a) and (c) number of proteins in the sample decreases specific activity decreases specific activity increases both (a) and (c) ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Affinity chromatography deals with the specific binding of a protein constituents for another molecule protein - carbohydrate interaction None of these protein - protein interaction specific binding of a protein constituents for another molecule protein - carbohydrate interaction None of these protein - protein interaction ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Proteins separation can be carried out on the basis of net charge size or mass all of these solubility in salt solutions net charge size or mass all of these solubility in salt solutions ANSWER DOWNLOAD EXAMIANS APP