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Transcription and Regulation

Transcription and Regulation
Which of the following is necessary for transcription to occur?

RNA polymerase
DNA polymerase
DNA molecule
Both DNA & RNA polymerase

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Transcription and Regulation
The closed complex at bacterial promoters is

bound more tightly when repressors are present
in equilibrium with free RNA polymerase and the promoter
not affected by activator proteins
not affected by promoter mutations

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Transcription and Regulation
Rho-dependent termination of transcription in E. coli

both (a) and (b)
removes mRNA and holoenzyme from the DNA
requires ATP
requires about 50 nucleotides of uncomplexed mRNA

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Transcription and Regulation
RNA polymerase holoenzyme initiates transcription which involves

recognition of the -10 sequence and -35 sequence
recognition of the -35 sequence only
None of these
recognition of the -10 sequence only

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Transcription and Regulation
Each tRNA molecule has a cloverleaf secondary structure consisting of

three stem loops
two stem loops
either (a) or (b)
four stem loops

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Transcription and Regulation
RNA polymerase in prokaryotes has a removable

alpha subunit
both (a) and (b)
beta subunit
sigma subunit

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