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Transcription and Regulation

Transcription and Regulation
Which of the following is necessary for transcription to occur?

Both DNA & RNA polymerase
DNA polymerase
RNA polymerase
DNA molecule

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Transcription and Regulation
The closed complex at bacterial promoters is

bound more tightly when repressors are present
not affected by activator proteins
not affected by promoter mutations
in equilibrium with free RNA polymerase and the promoter

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Transcription and Regulation
Rho-dependent termination of transcription in E. coli

requires about 50 nucleotides of uncomplexed mRNA
removes mRNA and holoenzyme from the DNA
both (a) and (b)
requires ATP

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Transcription and Regulation
In eukaryotes, there are three different RNA polymerases. The RNA polymerase responsible for transcription of mRNA is

RNA polymerase III
RNA polymerase I
none of these
RNA polymerase II

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Transcription and Regulation
Immediately after transcription

a methylated guanine cap is added to the 31 end of the transcript
a poly adenylation signal is added
a methylated guanine cap is added to the 51 end of the transcript
both (a) and (b)

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Transcription and Regulation
The RNA polymerases that transcribe bacterial DNA are

only active inside the cell
interchangable with DNA polymerases
multisubunit enzymes
highly glycosylated in their active forms

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