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Transcription and Regulation

Transcription and Regulation
RNA polymerase in prokaryotes has a removable

alpha subunit
beta subunit
both (a) and (b)
sigma subunit

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Transcription and Regulation
The closed complex at bacterial promoters is

not affected by activator proteins
bound more tightly when repressors are present
not affected by promoter mutations
in equilibrium with free RNA polymerase and the promoter

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Transcription and Regulation
The binding of lac repressor to DNA could be considered to be analogous to

competitive inhibition of an enzyme
allosteric effects in enzyme regulation
mixed-type inhibition of an enzyme
uncompetitive inhibition of an enzyme

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Transcription and Regulation
Rho-dependent termination of transcription in E. coli

removes mRNA and holoenzyme from the DNA
requires about 50 nucleotides of uncomplexed mRNA
both (a) and (b)
requires ATP

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Transcription and Regulation
Upstream regions which are essential for correct transcription are called

transcription factors
promoters
start codons
enhancers

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Transcription and Regulation
RNA polymerase holoenzyme initiates transcription which involves

None of these
recognition of the -10 sequence and -35 sequence
recognition of the -10 sequence only
recognition of the -35 sequence only

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