Transcription and Regulation
The closed complex at bacterial promoters is

not affected by activator proteins
in equilibrium with free RNA polymerase and the promoter
not affected by promoter mutations
bound more tightly when repressors are present

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Transcription and Regulation
The transcriptional regulatory proteins in eukaryotes which induce transcription usually have

at least two distinct domains of protein structure, a DNA binding domain and an activation domain
at least two distinct domains of protein structure and a DNA binding domain
at least one distinct domains of protein structure, a DNA binding domain and an activation domain
none of these

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