Transcription and Regulation
The closed complex at bacterial promoters is

in equilibrium with free RNA polymerase and the promoter
bound more tightly when repressors are present
not affected by activator proteins
not affected by promoter mutations

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Transcription and Regulation
Most transcripts of protein coding genes are cleaved

at the 5' end immediately before a poly adenylation signal
at the 3' end just after a poly adenylation signal
at the 5' end immediately after a poly adenylation signal
at the 3' end just before a poly adenylation signal

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Transcription and Regulation
Multiple copies of 5S genes, located at a chromosomal site distinct from the other rRNA genes

are transcribed by rRNA polymerase I and the 5S rRNA
are transcribed by DNA polymerase I
are transcribed by rRNA polymerase III and the 5S rRNA
are transcribed by rRNA polymerase II

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