Transcription and Regulation
The closed complex at bacterial promoters is

in equilibrium with free RNA polymerase and the promoter
not affected by activator proteins
bound more tightly when repressors are present
not affected by promoter mutations

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Transcription and Regulation
The major function of RNA polymerase's sigma factor is

recognition of the transcriptional stop sequence
recognition of the translational start sequence
recognition of the translational stop sequence
recognition of the transcriptional start sequence

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Transcription and Regulation
Most transcripts of protein coding genes are cleaved

at the 5' end immediately after a poly adenylation signal
at the 3' end just before a poly adenylation signal
at the 3' end just after a poly adenylation signal
at the 5' end immediately before a poly adenylation signal

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