Transcription and Regulation
The closed complex at bacterial promoters is

not affected by activator proteins
not affected by promoter mutations
in equilibrium with free RNA polymerase and the promoter
bound more tightly when repressors are present

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Transcription and Regulation
The major function of RNA polymerase's sigma factor is

recognition of the transcriptional start sequence
recognition of the translational stop sequence
recognition of the transcriptional stop sequence
recognition of the translational start sequence

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