Transcription and Regulation
The closed complex at bacterial promoters is

bound more tightly when repressors are present
not affected by promoter mutations
not affected by activator proteins
in equilibrium with free RNA polymerase and the promoter

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Transcription and Regulation
Most transcripts of protein coding genes are cleaved

at the 3' end just after a poly adenylation signal
at the 5' end immediately before a poly adenylation signal
at the 3' end just before a poly adenylation signal
at the 5' end immediately after a poly adenylation signal

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