Transcription and Regulation
The closed complex at bacterial promoters is

not affected by promoter mutations
in equilibrium with free RNA polymerase and the promoter
bound more tightly when repressors are present
not affected by activator proteins

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Transcription and Regulation
The function of the sigma factor of RNA polymerase is to

assure that translation begins at the proper point
assure that translation ends at the proper point
assure that transcription begins at the proper point
assure that transcription ends at the proper point

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