Transcription and Regulation
The closed complex at bacterial promoters is

not affected by promoter mutations
not affected by activator proteins
bound more tightly when repressors are present
in equilibrium with free RNA polymerase and the promoter

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Transcription and Regulation
The major function of RNA polymerase's sigma factor is

recognition of the transcriptional start sequence
recognition of the translational start sequence
recognition of the transcriptional stop sequence
recognition of the translational stop sequence

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