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VECTORS USES FOR ANIMAL CELL CULTURE

Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Over production of proteins of commercial value
Ability to clone large piece of DNA
All of these
Ability to glycosylate proteins during secretion

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Allows transformation by using crossing over
Carries centromere and telomere sequences
Carries autonomously replicating sequence
Has a origin of replication and rep genes

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Contains centromeric sequences
Functions as true chromosome
All of these
Segregates during meiosis and mitosis

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Vectors Uses for Animal Cell Culture
Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Vaccinia virus
Retrovirus
Papilloma virus
Baculo virus

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

essential for baculo virus replication
major component of protein matrix in which viruses are embedded
both (a) and (b)
non essential for baculo virus replication

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