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VECTORS USES FOR ANIMAL CELL CULTURE

Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Over production of proteins of commercial value
Ability to clone large piece of DNA
Ability to glycosylate proteins during secretion
All of these

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Has a origin of replication and rep genes
Allows transformation by using crossing over
Carries autonomously replicating sequence
Carries centromere and telomere sequences

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Functions as true chromosome
All of these
Contains centromeric sequences
Segregates during meiosis and mitosis

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Vectors Uses for Animal Cell Culture
Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Vaccinia virus
Papilloma virus
Retrovirus
Baculo virus

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

both (a) and (b)
non essential for baculo virus replication
major component of protein matrix in which viruses are embedded
essential for baculo virus replication

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