Protein Stability
Unpaired H-bond donors and acceptors are found in the hydrophobic core of a protein

rarely
only on Pro residues
only at the ends of a-helices
only at the turns connecting p-strands

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Protein Stability
Since ΔG° = -RTlnK

a 10-fold decrease in K decreases ΔG° by about 2.3*RT
a 10-fold increase in K decreases ΔG° by about 10-fold
a 10-fold increase in K decreases ΔG° by about 2.3*RT
a 10-fold decrease in K increases ΔG° by about 10-fold

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