Protein Stability
At the midpoint of a temperature transition curve,

Keq = 1.0 and ΔG = 0
half of the protein is denatured
All of these
[Native] = [Unfolded]

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Protein Stability
Since ΔG° = -RTlnK

a 10-fold increase in K decreases ΔG° by about 10-fold
a 10-fold decrease in K increases ΔG° by about 10-fold
a 10-fold decrease in K decreases ΔG° by about 2.3*RT
a 10-fold increase in K decreases ΔG° by about 2.3*RT

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