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Transcription and Regulation

Transcription and Regulation
The nucleons of eukaryotic cell has

RNA polymerase II
RNA polymerase I
all of these
RNA polymerase III

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Transcription and Regulation
In prokaryotes, transcription is

terminated by a poly A sequence
terminated by a protein called rho
terminated by the stop codon
terminated by a start codon

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Transcription and Regulation
Transcription factors are

proteins which bind to DNA and regulate transcription
promoters
TATA boxes
CATT boxes

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Transcription and Regulation
Immediately after transcription

a methylated guanine cap is added to the 31 end of the transcript
both (a) and (b)
a poly adenylation signal is added
a methylated guanine cap is added to the 51 end of the transcript

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Transcription and Regulation
Rho-dependent termination of transcription in E. coli

requires about 50 nucleotides of uncomplexed mRNA
both (a) and (b)
removes mRNA and holoenzyme from the DNA
requires ATP

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Transcription and Regulation
The RNA polymerases that transcribe bacterial DNA are

highly glycosylated in their active forms
only active inside the cell
interchangable with DNA polymerases
multisubunit enzymes

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