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Transcription and Regulation

Transcription and Regulation
Transcription factors are

TATA boxes
proteins which bind to DNA and regulate transcription
CATT boxes
promoters

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Transcription and Regulation
Enhancers are regions that

are adjacent to the TATA box
modulate transcription
bind RNA polymerase
are CAT box binding proteins

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Transcription and Regulation
RNA polymerase in prokaryotes has a removable

alpha subunit
sigma subunit
both (a) and (b)
beta subunit

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Transcription and Regulation
Rho-dependent termination of transcription in E. coli

requires ATP
removes mRNA and holoenzyme from the DNA
requires about 50 nucleotides of uncomplexed mRNA
both (a) and (b)

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Transcription and Regulation
The binding of lac repressor to DNA could be considered to be analogous to

competitive inhibition of an enzyme
uncompetitive inhibition of an enzyme
mixed-type inhibition of an enzyme
allosteric effects in enzyme regulation

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Transcription and Regulation
The regions of a gene located at -10 and -35, with respect to the start of transcription, are termed

start codons.
promoters.
Shine Dalgarno sequences.
oriC regions.

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