Microbiology of Soils
The nonsymbiotic bacteria which fix nitrogen live in the soil independently are

All of these
Clostridium
Azotobacter
considerably less important in comparison to the symbiotic bacteria

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Microbiology of Soils
Nitrogen fixation by the microorganisms can be detected by adopting the approach of

All of these
measuring15N2 by mass spectrometer
demonstrating growth in a nitrogen free medium
cultivating the microorganisms in the presence of nitrogen labeled with isotropic nitrogen

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