Allosteric Effects
Small molecules affect hemoglobin (Hb) by

increasing [H+]
increasing Hb affinity for O2
increasing [H+] and decreasing Hb affinity for O2
decreasing Hb affinity for O2

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Allosteric Effects
O2 binding to hemoglobin results in

100-fold lower affinity for the last O2 bound than for the first
100-fold higher affinity for the last O2 bound than for the first
extensive protein conformational change
both (a) and (b)

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