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PROTEIN PURIFICATION

Protein Purification
A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

1,000,000 units/mg
1,000 units/mg
10,000 units/mg.
100,000 units/mg

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Protein Purification
Proteins separation can be carried out on the basis of

solubility in salt solutions
all of these
size or mass
net charge

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Protein Purification
The best way to determine the location of protein in the purification scheme is to measure the

rate of ATP synthesis
mass spectroscopy of the protein
changes in the refractive index
UV absorption

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