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Polymerase Chain Reaction in Biochemistry

Polymerase Chain Reaction in Biochemistry
PCR (Polymerase Chain Reaction) can make multiple copies of

carbohydrate
DNA
protein
RNA

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Polymerase Chain Reaction in Biochemistry
PCR is used

to solve crimes
to diagnose genetic diseases
all of these
to study gene function

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Polymerase Chain Reaction in Biochemistry
In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to

18 single strands of DNA
16 double stranded DNA
18 double stranded DNA
16 single strands of DNA

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Polymerase Chain Reaction in Biochemistry
Transformation means

F+ and F- strains of bacteria
acquiring DNA from the bacterial cell environment
formation of a pilus
plasmid containing a F factor

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Polymerase Chain Reaction in Biochemistry
For gene transfer to be effective, transforming DNA must be

incorporated into a viral genome
None of these
incorporated into the bacterial chromosome
free in the bacterial cytoplasm

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Polymerase Chain Reaction in Biochemistry
What technique can be used to determine the murderer who left blood with the victim?

DNA sequencing
Western blot
RFLP mapping
PCR amplification

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