Polymerase Chain Reaction in Biochemistry
To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

with different restriction enzyme and mixed together
with the combination of enzymes and mixed together
with the same restriction enzyme and mixed together
with the combination of enzymes and then seperated

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Polymerase Chain Reaction in Biochemistry
Restriction maps

None of these
does not allow comparison between DNA molecules
allows comparison between DNA molecules but requires to determine nucleotide sequence
allows comparison between DNA molecules without the need to determine nucleotide sequence

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Polymerase Chain Reaction in Biochemistry
Southern blotting involves

electrophoresis of DNA molecules followed by incubation
electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation
electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation

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Polymerase Chain Reaction in Biochemistry
A PCR cycle consists of

three steps, initiation, elongation and termination
three steps, primer annealing, elongation and termination
three steps, denaturation, primer annealing and elongation
three steps, denaturation, initiation and elongation

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