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Polymerase Chain Reaction in Biochemistry

Polymerase Chain Reaction in Biochemistry
Double stranded DNA denaturation with specified limit of temperature is

reversible reaction
either (a) or (b)
none of these
irreversible reaction

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Polymerase Chain Reaction in Biochemistry
In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to

18 double stranded DNA
16 single strands of DNA
16 double stranded DNA
18 single strands of DNA

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Polymerase Chain Reaction in Biochemistry
Additional mono saccharides are added in Golgi to produce

either high sucrose type polysaccharide or a complex type
either fructose type monosaccharide or a complex type
either high mannose type oligosaccharide or a complex type
None of these

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Polymerase Chain Reaction in Biochemistry
Transformation means

acquiring DNA from the bacterial cell environment
plasmid containing a F factor
formation of a pilus
F+ and F- strains of bacteria

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Polymerase Chain Reaction in Biochemistry
PCR can be used to amplify a specific fragment of DNA from which of the following?

All of these
A drop of blood
A fragment of skin
A hair follicle

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Polymerase Chain Reaction in Biochemistry
For gene transfer to be effective, transforming DNA must be

free in the bacterial cytoplasm
incorporated into the bacterial chromosome
None of these
incorporated into a viral genome

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