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Polymerase Chain Reaction in Biochemistry

Polymerase Chain Reaction in Biochemistry
Double stranded DNA denaturation with specified limit of temperature is

irreversible reaction
either (a) or (b)
reversible reaction
none of these

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Polymerase Chain Reaction in Biochemistry
Transformation means

acquiring DNA from the bacterial cell environment
F+ and F- strains of bacteria
formation of a pilus
plasmid containing a F factor

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Polymerase Chain Reaction in Biochemistry
DNA fragments in a restriction digest can be separated by electrophoresis in

agarose gel
poly acrylamide
none of these
both (a) and (b)

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Polymerase Chain Reaction in Biochemistry
From a single molecule of DNA, PCR can make

one additional copy
millions of copies
thousands of copies
hundreds of copies

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Polymerase Chain Reaction in Biochemistry
PCR is used

to diagnose genetic diseases
to study gene function
all of these
to solve crimes

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Polymerase Chain Reaction in Biochemistry
For gene transfer to be effective, transforming DNA must be

free in the bacterial cytoplasm
incorporated into the bacterial chromosome
incorporated into a viral genome
None of these

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