Polymerase Chain Reaction in Biochemistry
A PCR cycle consists of

three steps, denaturation, primer annealing and elongation
three steps, denaturation, initiation and elongation
three steps, primer annealing, elongation and termination
three steps, initiation, elongation and termination

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Polymerase Chain Reaction in Biochemistry
To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

with the same restriction enzyme and mixed together
with the combination of enzymes and mixed together
with different restriction enzyme and mixed together
with the combination of enzymes and then seperated

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