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Polymerase Chain Reaction in Biochemistry

Polymerase Chain Reaction in Biochemistry
Genomic libraries are made from

genomic cDNA of an organism
genomic DNA of an organism
genomic RNA of an organism
genomic mRNA of an organism

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Polymerase Chain Reaction in Biochemistry
How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?

Eight ds DNA molecule
Two ds DNA molecule
Four ds DNA molecule
Three ds DNA molecule

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Polymerase Chain Reaction in Biochemistry
For gene transfer to be effective, transforming DNA must be

incorporated into a viral genome
incorporated into the bacterial chromosome
None of these
free in the bacterial cytoplasm

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Polymerase Chain Reaction in Biochemistry
Transformation means

F+ and F- strains of bacteria
acquiring DNA from the bacterial cell environment
formation of a pilus
plasmid containing a F factor

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Polymerase Chain Reaction in Biochemistry
Double stranded DNA denaturation with specified limit of temperature is

either (a) or (b)
none of these
irreversible reaction
reversible reaction

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Polymerase Chain Reaction in Biochemistry
The PCR, polymerase chain reaction is becoming the method of choice for

sterilization of gene
screening gene
Alteration of gene
all of these

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