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Polymerase Chain Reaction in Biochemistry

Polymerase Chain Reaction in Biochemistry
PCR amplifies millions of copies of

short chain RNA
a short fragment of DNA
the entire genome (all the DNA in a sample)
both (a) and (b)

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Polymerase Chain Reaction in Biochemistry
DNA fragments in a restriction digest can be separated by electrophoresis in

poly acrylamide
both (a) and (b)
agarose gel
none of these

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Polymerase Chain Reaction in Biochemistry
PCR (Polymerase Chain Reaction) can make multiple copies of

protein
DNA
RNA
carbohydrate

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Polymerase Chain Reaction in Biochemistry
Transformation means

acquiring DNA from the bacterial cell environment
F+ and F- strains of bacteria
plasmid containing a F factor
formation of a pilus

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Polymerase Chain Reaction in Biochemistry
In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to

18 double stranded DNA
18 single strands of DNA
16 double stranded DNA
16 single strands of DNA

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Polymerase Chain Reaction in Biochemistry
For gene transfer to be effective, transforming DNA must be

None of these
incorporated into the bacterial chromosome
incorporated into a viral genome
free in the bacterial cytoplasm

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