Polymerase Chain Reaction
Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?

All of these
Mycobacterium tuberculosis
Hepatitis B virus
HIV-1 and HIV-2 viruses

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

reduction in substrate concentration
all of these
insufficient enzyme and time to synthesize mass quantity of DNA
build up of PCR product which competes with primers for hybrid formation

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