Polymerase Chain Reaction
The cloning step in PCR/sequencing analysis of microbial communities is necessary for

preventing contamination by outside DNA
the amplification process
separating the different rRNA gene sequences in the mixture
none of these

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

insufficient enzyme and time to synthesize mass quantity of DNA
reduction in substrate concentration
build up of PCR product which competes with primers for hybrid formation
all of these

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