Polymerase Chain Reaction
The cloning step in PCR/sequencing analysis of microbial communities is necessary for

none of these
separating the different rRNA gene sequences in the mixture
preventing contamination by outside DNA
the amplification process

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

build up of PCR product which competes with primers for hybrid formation
insufficient enzyme and time to synthesize mass quantity of DNA
all of these
reduction in substrate concentration

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