Polymerase Chain Reaction
The cloning step in PCR/sequencing analysis of microbial communities is necessary for

the amplification process
separating the different rRNA gene sequences in the mixture
preventing contamination by outside DNA
none of these

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

build up of PCR product which competes with primers for hybrid formation
reduction in substrate concentration
all of these
insufficient enzyme and time to synthesize mass quantity of DNA

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