Polymerase Chain Reaction
The cloning step in PCR/sequencing analysis of microbial communities is necessary for

none of these
the amplification process
preventing contamination by outside DNA
separating the different rRNA gene sequences in the mixture

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

build up of PCR product which competes with primers for hybrid formation
reduction in substrate concentration
insufficient enzyme and time to synthesize mass quantity of DNA
all of these

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