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Protein and Nucleic Acid Interactions

Protein and Nucleic Acid Interactions
Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

Ile and Val
Cys and Met
Lys and Leu
Asn and Gln

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Protein and Nucleic Acid Interactions
The type II restriction endonucleases, bind to DNA sites

All of these
using H-bonds for specificity
that are usually palindromic
using non-sequence-dependent backbone interactions

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Protein and Nucleic Acid Interactions
Cleavage of DNA by EcoRI endonuclease results in the formation of

one 3'-OH end and one 5'-phosphate end
one 3'-OH end and two 5'-phosphate end
two 3'-OH ends and two 5'-phosphate ends
two 3'-phosphate ends and two 3'-OH ends

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Protein and Nucleic Acid Interactions
Reverse transcripase from HIV does not

has a 3'- 5' exonuclease activity
requires a primer
uses RNA as a template
has a 5' to 3' polymerase activity

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Protein and Nucleic Acid Interactions
Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

Cys and Met
Lys and Arg
Ile and Val
Leu and Ala

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Protein and Nucleic Acid Interactions
The enzyme that joins DNA cuts is called

ligase
reverse transcriptase
joinase
DNA phosphorylase

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