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Plant Cell Culture

Plant Cell Culture
When plated only in nutrient medium, how much time is required for the protoplast to synthesize new cell wall?

5-10 days
10-15 days
2-5 days
15-17 days

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Plant Cell Culture
Which of the following is used in the culture of regenerating protoplasts, single cells or very dilute cell suspensions?

Both (a) and (b)
None of these
Nurse or feeder culture
Nurse medium

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Plant Cell Culture
Cellular totipotency is the property of

all of these
animals
bacteria
plants

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Plant Cell Culture
Which of the following is considered as the disadvantage of conventional plant tissue culture for clonal propagation?

Storage and transportation of propogates
Multiplication of sexually derived sterile hybrids
Both (b) and (c)
Less multiplication of disease free plants

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Plant Cell Culture
Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

proteolytic enzymes
cellulotyic enzymes
pectolytic enzymes
both cellulotyic and pectolytic enzymes

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Plant Cell Culture
Which method of plant propagation involves the use of girdling?

Micropropagation
Grafting
Layering
Cuttings

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