Plant Cell Culture
Subculturing is similar to propagation by cuttings because

they both use in vitro growing conditions
it separates multiple microshoots and places them in a medium
it uses scions to produce new microshoots
All of these

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Plant Cell Culture
In a callus culture

increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
only auxin is required for root and shoot formation
auxins and cytokinins are not required
increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation

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