Transcription
The location of first hexameric sequence typically found in pro-karyotic promoters is

at the transcription start site
approximately 35 bases upstream of the transcription start site
approximately 25 bases upstream of the transcription start site
approximately 10 bases upstream of the transcription start site

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Transcription
The sigma factor

accomplishes DNA restriction
is responsible for recognizing the termination sequence
recognizes the promotor
provides the catalytic function of cleaving off pyrophosphates during mRNA synthesis

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