Transcription
The location of first hexameric sequence typically found in pro-karyotic promoters is

approximately 10 bases upstream of the transcription start site
approximately 25 bases upstream of the transcription start site
approximately 35 bases upstream of the transcription start site
at the transcription start site

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Transcription
The sigma factor

recognizes the promotor
is responsible for recognizing the termination sequence
provides the catalytic function of cleaving off pyrophosphates during mRNA synthesis
accomplishes DNA restriction

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