Transcription
The location of first hexameric sequence typically found in pro-karyotic promoters is

approximately 25 bases upstream of the transcription start site
at the transcription start site
approximately 10 bases upstream of the transcription start site
approximately 35 bases upstream of the transcription start site

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Transcription
The sigma factor

is responsible for recognizing the termination sequence
recognizes the promotor
accomplishes DNA restriction
provides the catalytic function of cleaving off pyrophosphates during mRNA synthesis

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