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Transcription and Regulation

Transcription and Regulation
The regions of a gene located at -10 and -35, with respect to the start of transcription, are termed

promoters.
start codons.
Shine Dalgarno sequences.
oriC regions.

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Transcription and Regulation
RNA is synthesized on a DNA template in a process called __________ , which utilizes the enzyme __________

transcription, RNA polymerase
translation, RNA polymerase
transcription, DNA polymerase
replication, DNA polymerase

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Transcription and Regulation
Rho-dependent termination of transcription in E. coli

removes mRNA and holoenzyme from the DNA
both (a) and (b)
requires about 50 nucleotides of uncomplexed mRNA
requires ATP

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Transcription and Regulation
The closed complex at bacterial promoters is

in equilibrium with free RNA polymerase and the promoter
not affected by activator proteins
not affected by promoter mutations
bound more tightly when repressors are present

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Transcription and Regulation
The RNA polymerases that transcribe bacterial DNA are

only active inside the cell
interchangable with DNA polymerases
highly glycosylated in their active forms
multisubunit enzymes

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Transcription and Regulation
Transcription by E. coli polymerase occurs in

four phases known as initiation, propagation, elongation and termination
None of these
three phases known as initiation, elongation and termination
two phases known as initiation and termination

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