Microbial Metabolism
The major route for incorporation of ammonia (NH4+) into organic compounds is via

oxidation of pyruvate
atmospheric nitrogen fixation
all of these
reduction of pyruvate or alpha-ketoglutarate by enzymes

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Microbial Metabolism
The mechanism of passive or facilitated diffusion require

accumulation of solute against an electrochemical gradient
accumulation or concentration of solute against an electrochemical gradient
concentration of solute against an electrochemical gradient
metabolic energy

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