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Protein and Nucleic Acid Interactions

Protein and Nucleic Acid Interactions
Reverse transcripase from HIV does not

uses RNA as a template
requires a primer
has a 3'- 5' exonuclease activity
has a 5' to 3' polymerase activity

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Protein and Nucleic Acid Interactions
The type II restriction endonucleases, bind to DNA sites

using non-sequence-dependent backbone interactions
using H-bonds for specificity
All of these
that are usually palindromic

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Protein and Nucleic Acid Interactions
Cleavage of DNA by EcoRI endonuclease results in the formation of

one 3'-OH end and one 5'-phosphate end
two 3'-phosphate ends and two 3'-OH ends
two 3'-OH ends and two 5'-phosphate ends
one 3'-OH end and two 5'-phosphate end

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Protein and Nucleic Acid Interactions
Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

negative direction
5' to 3' direction
3' to 5' direction
positive direction

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Protein and Nucleic Acid Interactions
The enzyme that joins DNA cuts is called

DNA phosphorylase
joinase
reverse transcriptase
ligase

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Protein and Nucleic Acid Interactions
Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

Leu and Ala
Lys and Arg
Cys and Met
Ile and Val

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