VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

12μm yeast plasmid and E coli vector pBR 322

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

pBR 322 vector

none of these

SV 40 helper virus

COS cell line

Functions as true chromosome

Contains centromeric sequences

All of these

Segregates during meiosis and mitosis

with the RNA mediated transfection

with the DNA mediated transfection

without the DNA mediated transfection

without the RNA mediated transfection

Ura 3

All of these

His 3

Trp 1

Trp 1

Ura 3

His 3

Lys 2

Bovine papilloma virus (BVP)

SV 40 virus

Retroviruses

All of these

Trp 1

His 3

Lys 2

Ura 3

is inserted between the polyhedrin promoter and downstream sequences

is inserted randomly in viral genome

is inserted downstream of viral origin of replication

is inserted downstream of polyhedrin promoter

not necessary for viral replication

coding for virus coat protein

important for packaging of viral DNA into viral head

transcriptional regulators and are essential for viral replication