VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

H1

H3

H4

H2A

110 kb

7.2 kb

5.2 kb

69 kb

All of these

Ability to glycosylate proteins during secretion

Over production of proteins of commercial value

Ability to clone large piece of DNA

5 kb

10 kb

1 kb

2.5 kb

Segregates during meiosis and mitosis

Functions as true chromosome

Contains centromeric sequences

All of these

important for packaging of viral DNA into viral head

not necessary for viral replication

transcriptional regulators and are essential for viral replication

coding for virus coat protein

Vaccinia virus

Baculo virus

Retrovirus

Papilloma virus

Phosphoglycerate kinase (PGK)

Galactokinase (GAL 1)

Acid phosphatase (PHOS)

Both (a) and (b)

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

12μm yeast plasmid and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

integrated only

either episomally or integrated at low copy number

episomally only

either episomally or integrated at high copy number