VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

5.2 kb

2.5 kb

7.9 kb

9.5 kb

viral gene expression is driven by strong promoters

All of these

they cover a wide host range

infection does not lead to cell death, infected cells produce virus over an indefinite period

4-6 kb

20 kb

6-8 kb

10-12 kb

cohesive initial site of phage λ

derivative of non-permissive CV-1 monkey cell line

cohesive end site of phage λ

derivative of permissive CV-1 monkey cell line

69 kb

5.2 kb

7.2 kb

110 kb

Baculo virus

none of these

Vaccinia virus

Retro virus

Allows transformation by using crossing over

Carries centromere and telomere sequences

Carries autonomously replicating sequence

Has a origin of replication and rep genes

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

12μm yeast plasmid and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

both (a) and (c)

telomere

centromere

autonomously replicating sequence

telomere

all of these

autonomously replicating sequence

centromere