VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1

Lys 2

Ura 3

His 3

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Yeast episomal plasmids (YEp) were constructed using

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

12μm yeast plasmid and E coli vector pBR 322

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YAC vectors can be used to obtain artificial chromosomes because it carries

autonomously replicating sequence

telomere

centromere

all of these

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Which one of the following is the most stable vector?

YEp

YIp

YAC

YRp

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Which of the following(s) is/are the most commonly used markers in yeast?

All of these

His 3

Trp 1

Ura 3

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Which of the following viruses are used as vectors for transferring genes to animal cells?

Retroviruses

Bovine papilloma virus (BVP)

All of these

SV 40 virus

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Maximum amount of foreign DNA introduced into SV 40 viruses is

1 kb

10 kb

2.5 kb

5 kb

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Which of the following histone is not present in SV40 virus?

H3

H2A

H4

H1

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Which of the following is/are commonly used promoter of S. cerevisiae?

Phosphoglycerate kinase (PGK)

Galactokinase (GAL 1)

Alcohol dehydrogenase II (ADH 2)

All of these

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Cloning genes in the yeast offer which of following advantage(s)?

All of these

Ability to glycosylate proteins during secretion

Over production of proteins of commercial value

Ability to clone large piece of DNA

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