VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

4-6 kb

20 kb

6-8 kb

10-12 kb

Lys 2

Ura 3

Trp 1

His 3

Ss DNA genome

cDNA genome

RNA genome

Ds DNA genome

Galactokinase (GAL 1)

All of these

Phosphoglycerate kinase (PGK)

Alcohol dehydrogenase II (ADH 2)

YCp

YIp

YRp

YAC

derivative of permissive CV-1 monkey cell line

cohesive end site of phage λ

cohesive initial site of phage λ

derivative of non-permissive CV-1 monkey cell line

without the DNA mediated transfection

without the RNA mediated transfection

with the RNA mediated transfection

with the DNA mediated transfection

all of these

autonomously replicating sequence

telomere

centromere

All of these

viral gene expression is driven by strong promoters

infection does not lead to cell death, infected cells produce virus over an indefinite period

they cover a wide host range

12μm yeast plasmid and E coli vector pMB 9

12μm yeast plasmid and E coli vector pBR 322

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9