VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

either episomally or integrated at low copy number

either episomally or integrated at high copy number

episomally only

integrated only

both (a) and (b)

non essential for baculo virus replication

major component of protein matrix in which viruses are embedded

essential for baculo virus replication

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

12μm yeast plasmid and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

Ura 3

His 3

Lys 2

Trp 1

YRp

YIp

YCp

YAC

YEp

YCp

Both (a) and (b)

YRp

H4

H2A

H3

H1

Over production of proteins of commercial value

Ability to glycosylate proteins during secretion

All of these

Ability to clone large piece of DNA

without the DNA mediated transfection

without the RNA mediated transfection

with the DNA mediated transfection

with the RNA mediated transfection

Has a origin of replication and rep genes

Carries centromere and telomere sequences

Allows transformation by using crossing over

Carries autonomously replicating sequence