VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

they cover a wide host range

infection does not lead to cell death, infected cells produce virus over an indefinite period

All of these

viral gene expression is driven by strong promoters

pBR 322 vector

none of these

SV 40 helper virus

COS cell line

non essential for baculo virus replication

both (a) and (b)

essential for baculo virus replication

major component of protein matrix in which viruses are embedded

20 kb

10-12 kb

6-8 kb

4-6 kb

12μm yeast plasmid and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

Trp 1

Lys 2

Ura 3

His 3

All of these

Over production of proteins of commercial value

Ability to glycosylate proteins during secretion

Ability to clone large piece of DNA

Alcohol dehydrogenase II (ADH 2)

Galactokinase (GAL 1)

All of these

Phosphoglycerate kinase (PGK)

Carries centromere and telomere sequences

Carries autonomously replicating sequence

Allows transformation by using crossing over

Has a origin of replication and rep genes

Carries autonomously replicating sequence

Has a origin of replication and rep genes

Allows transformation by using crossing over

Carries centromere and telomere sequences