VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

2.5 kb

9.5 kb

5.2 kb

7.9 kb

derivative of non-permissive CV-1 monkey cell line

cohesive initial site of phage λ

cohesive end site of phage λ

derivative of permissive CV-1 monkey cell line

110 kb

5.2 kb

69 kb

7.2 kb

with the DNA mediated transfection

with the RNA mediated transfection

without the DNA mediated transfection

without the RNA mediated transfection

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

12μm yeast plasmid and E coli vector pBR 322

Trp 1

Lys 2

Ura 3

His 3

either episomally or integrated at high copy number

either episomally or integrated at low copy number

episomally only

integrated only

YRp

YIp

YAC

YEp

H4

H3

H2A

H1

Allows transformation by using crossing over

Has a origin of replication and rep genes

Carries autonomously replicating sequence

Carries centromere and telomere sequences