VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Which of the following histone is not present in SV40 virus?

H1

H4

H2A

H3

TRUE ANSWER : ?
YOUR ANSWER : ?

In case of Baculoviruses, the foreign gene

is inserted between the polyhedrin promoter and downstream sequences

is inserted randomly in viral genome

is inserted downstream of viral origin of replication

is inserted downstream of polyhedrin promoter

TRUE ANSWER : ?
YOUR ANSWER : ?

Cos cell line is

derivative of non-permissive CV-1 monkey cell line

cohesive end site of phage λ

derivative of permissive CV-1 monkey cell line

cohesive initial site of phage λ

TRUE ANSWER : ?
YOUR ANSWER : ?

Retroviruses have advantage for being used as vector for animal cells because

viral gene expression is driven by strong promoters

they cover a wide host range

infection does not lead to cell death, infected cells produce virus over an indefinite period

All of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Retroviruses consist of

Ss DNA genome

RNA genome

cDNA genome

Ds DNA genome

TRUE ANSWER : ?
YOUR ANSWER : ?

Autonomously replicating sequence is not present in

YCp

YAC

YRp

YIp

TRUE ANSWER : ?
YOUR ANSWER : ?

Moloney-Mu-leukemia virus is an example of

none of these

Baculo virus

Retro virus

Vaccinia virus

TRUE ANSWER : ?
YOUR ANSWER : ?

SV 40 vectors, in which late region replacements are done, require

SV 40 helper virus

pBR 322 vector

COS cell line

none of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Cloning genes in the yeast offer which of following advantage(s)?

Ability to glycosylate proteins during secretion

Over production of proteins of commercial value

All of these

Ability to clone large piece of DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

The size of the genome of SV 40 is

7.2 kb

69 kb

5.2 kb

110 kb

TRUE ANSWER : ?
YOUR ANSWER : ?