VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Retrovirus

Vaccinia virus

Baculo virus

Papilloma virus

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Which one of the following is the most stable vector?

YIp

YAC

YRp

YEp

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Yeast Replicating plasmids (YRp) carry

both (a) and (c)

autonomously replicating sequence

telomere

centromere

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Retroviruses have advantage for being used as vector for animal cells because

infection does not lead to cell death, infected cells produce virus over an indefinite period

viral gene expression is driven by strong promoters

All of these

they cover a wide host range

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YAC vectors can be used to obtain artificial chromosomes because it carries

centromere

autonomously replicating sequence

all of these

telomere

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Autonomously replicating sequence is not present in

YRp

YCp

YIp

YAC

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Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Contains centromeric sequences

Functions as true chromosome

Segregates during meiosis and mitosis

All of these

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Which of the following viruses are used as vectors for transferring genes to animal cells?

Retroviruses

SV 40 virus

Bovine papilloma virus (BVP)

All of these

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α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

His 3

Lys 2

Trp 1

Ura 3

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In case of Baculoviruses, the foreign gene

is inserted downstream of polyhedrin promoter

is inserted downstream of viral origin of replication

is inserted between the polyhedrin promoter and downstream sequences

is inserted randomly in viral genome

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