VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Autonomously replicating sequence is not present in

YAC

YRp

YIp

YCp

TRUE ANSWER : ?
YOUR ANSWER : ?

Moloney-Mu-leukemia virus is an example of

Baculo virus

none of these

Vaccinia virus

Retro virus

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following promoters is repressed in presence of glucose?

Both (a) and (b)

Phosphoglycerate kinase (PGK)

Galactokinase (GAL 1)

Acid phosphatase (PHOS)

TRUE ANSWER : ?
YOUR ANSWER : ?

Retroviruses have advantage for being used as vector for animal cells because

infection does not lead to cell death, infected cells produce virus over an indefinite period

they cover a wide host range

All of these

viral gene expression is driven by strong promoters

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following are produced in high copy number?

Both (a) and (b)

YRp

YCp

YEp

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following(s) is/are the most commonly used markers in yeast?

Trp 1

Ura 3

His 3

All of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Bovine papilloma virus has a circular double stranded DNA genome of about

5.2 kb

7.9 kb

9.5 kb

2.5 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

Maximum insert size in case of adenovirus vectors is

4-6 kb

20 kb

10-12 kb

6-8 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

Retroviruses consist of

RNA genome

Ds DNA genome

Ss DNA genome

cDNA genome

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following can be used for reducing the size of gene inserts containing introns?

Baculo virus

none of these

Retro virus

Vaccinia virus

TRUE ANSWER : ?
YOUR ANSWER : ?