VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Which of the following is/are commonly used promoter of S. cerevisiae?

Alcohol dehydrogenase II (ADH 2)

Galactokinase (GAL 1)

Phosphoglycerate kinase (PGK)

All of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Retroviruses consist of

RNA genome

Ds DNA genome

Ss DNA genome

cDNA genome

TRUE ANSWER : ?
YOUR ANSWER : ?

YAC vectors can be used to obtain artificial chromosomes because it carries

all of these

telomere

centromere

autonomously replicating sequence

TRUE ANSWER : ?
YOUR ANSWER : ?

Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Has a origin of replication and rep genes

Allows transformation by using crossing over

Carries centromere and telomere sequences

Carries autonomously replicating sequence

TRUE ANSWER : ?
YOUR ANSWER : ?

SV 40 vectors, in which late region replacements are done, require

none of these

COS cell line

SV 40 helper virus

pBR 322 vector

TRUE ANSWER : ?
YOUR ANSWER : ?

5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

His 3

Lys 2

Trp 1

Ura 3

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following viruses are used as vectors for transferring genes to animal cells?

All of these

Bovine papilloma virus (BVP)

SV 40 virus

Retroviruses

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following promoters is repressed in presence of glucose?

Phosphoglycerate kinase (PGK)

Both (a) and (b)

Acid phosphatase (PHOS)

Galactokinase (GAL 1)

TRUE ANSWER : ?
YOUR ANSWER : ?

Polyhedrin protein is

essential for baculo virus replication

both (a) and (b)

non essential for baculo virus replication

major component of protein matrix in which viruses are embedded

TRUE ANSWER : ?
YOUR ANSWER : ?

Recombinant SV40 viruses introduce the foreign DNA into cells

without the DNA mediated transfection

with the DNA mediated transfection

with the RNA mediated transfection

without the RNA mediated transfection

TRUE ANSWER : ?
YOUR ANSWER : ?

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