VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Autonomously replicating sequence is not present in

YCp

YRp

YAC

YIp

TRUE ANSWER : ?
YOUR ANSWER : ?

Yeast Replicating plasmids (YRp) carry

centromere

autonomously replicating sequence

telomere

both (a) and (c)

TRUE ANSWER : ?
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Polyhedrin protein is

non essential for baculo virus replication

major component of protein matrix in which viruses are embedded

essential for baculo virus replication

both (a) and (b)

TRUE ANSWER : ?
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Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Vaccinia virus

Baculo virus

Papilloma virus

Retrovirus

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Which one of the following is the most stable vector?

YRp

YEp

YIp

YAC

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following promoters is repressed in presence of glucose?

Phosphoglycerate kinase (PGK)

Acid phosphatase (PHOS)

Both (a) and (b)

Galactokinase (GAL 1)

TRUE ANSWER : ?
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α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3

His 3

Lys 2

Trp 1

TRUE ANSWER : ?
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Retroviruses consist of

RNA genome

Ss DNA genome

Ds DNA genome

cDNA genome

TRUE ANSWER : ?
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Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at low copy number

episomally only

either episomally or integrated at high copy number

integrated only

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SV 40 vectors, in which late region replacements are done, require

COS cell line

pBR 322 vector

none of these

SV 40 helper virus

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