VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

His 3

Trp 1

Lys 2

Ura 3

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In case of Baculoviruses, the foreign gene

is inserted between the polyhedrin promoter and downstream sequences

is inserted downstream of viral origin of replication

is inserted randomly in viral genome

is inserted downstream of polyhedrin promoter

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Which of the following viruses are used as vectors for transferring genes to animal cells?

Bovine papilloma virus (BVP)

Retroviruses

SV 40 virus

All of these

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SV 40 vectors, in which early region replacements are carried out, require

COS cell line

none of these

SV 40 helper virus

pBR 322 vector

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Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Vaccinia virus

Retrovirus

Papilloma virus

Baculo virus

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Which of the following(s) is/are the most commonly used markers in yeast?

Trp 1

His 3

All of these

Ura 3

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Which one of the following is the most stable vector?

YAC

YIp

YEp

YRp

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Maximum amount of foreign DNA introduced into SV 40 viruses is

10 kb

5 kb

1 kb

2.5 kb

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Polyhedrin protein is

essential for baculo virus replication

non essential for baculo virus replication

major component of protein matrix in which viruses are embedded

both (a) and (b)

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Which of the following can be used for reducing the size of gene inserts containing introns?

Vaccinia virus

none of these

Retro virus

Baculo virus

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