VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Moloney-Mu-leukemia virus is an example of

Retro virus

Baculo virus

none of these

Vaccinia virus

TRUE ANSWER : ?
YOUR ANSWER : ?

Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries autonomously replicating sequence

Carries centromere and telomere sequences

Has a origin of replication and rep genes

Allows transformation by using crossing over

TRUE ANSWER : ?
YOUR ANSWER : ?

Cos cell line is

cohesive end site of phage λ

cohesive initial site of phage λ

derivative of permissive CV-1 monkey cell line

derivative of non-permissive CV-1 monkey cell line

TRUE ANSWER : ?
YOUR ANSWER : ?

Retroviruses have advantage for being used as vector for animal cells because

viral gene expression is driven by strong promoters

they cover a wide host range

All of these

infection does not lead to cell death, infected cells produce virus over an indefinite period

TRUE ANSWER : ?
YOUR ANSWER : ?

Maximum amount of foreign DNA introduced into SV 40 viruses is

2.5 kb

5 kb

10 kb

1 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

Polyhedrin protein is

major component of protein matrix in which viruses are embedded

both (a) and (b)

essential for baculo virus replication

non essential for baculo virus replication

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following can be used for reducing the size of gene inserts containing introns?

Baculo virus

Retro virus

none of these

Vaccinia virus

TRUE ANSWER : ?
YOUR ANSWER : ?

Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

transcriptional regulators and are essential for viral replication

not necessary for viral replication

important for packaging of viral DNA into viral head

coding for virus coat protein

TRUE ANSWER : ?
YOUR ANSWER : ?

SV 40 vectors, in which late region replacements are done, require

SV 40 helper virus

pBR 322 vector

COS cell line

none of these

TRUE ANSWER : ?
YOUR ANSWER : ?

5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Lys 2

Ura 3

His 3

Trp 1

TRUE ANSWER : ?
YOUR ANSWER : ?