VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Retro virus

Vaccinia virus

Baculo virus

none of these

not necessary for viral replication

important for packaging of viral DNA into viral head

transcriptional regulators and are essential for viral replication

coding for virus coat protein

Carries autonomously replicating sequence

Has a origin of replication and rep genes

Carries centromere and telomere sequences

Allows transformation by using crossing over

Bovine papilloma virus (BVP)

SV 40 virus

Retroviruses

All of these

infection does not lead to cell death, infected cells produce virus over an indefinite period

viral gene expression is driven by strong promoters

they cover a wide host range

All of these

Acid phosphatase (PHOS)

Both (a) and (b)

Phosphoglycerate kinase (PGK)

Galactokinase (GAL 1)

Functions as true chromosome

Contains centromeric sequences

Segregates during meiosis and mitosis

All of these

6-8 kb

4-6 kb

20 kb

10-12 kb

Trp 1

Lys 2

Ura 3

His 3

episomally only

either episomally or integrated at low copy number

integrated only

either episomally or integrated at high copy number