VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Which of the following promoters is repressed in presence of glucose?

Both (a) and (b)

Galactokinase (GAL 1)

Acid phosphatase (PHOS)

Phosphoglycerate kinase (PGK)

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following histone is not present in SV40 virus?

H4

H2A

H3

H1

TRUE ANSWER : ?
YOUR ANSWER : ?

Maximum amount of foreign DNA introduced into SV 40 viruses is

1 kb

5 kb

2.5 kb

10 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following(s) is/are the most commonly used markers in yeast?

Trp 1

All of these

Ura 3

His 3

TRUE ANSWER : ?
YOUR ANSWER : ?

Maximum insert size in case of adenovirus vectors is

6-8 kb

20 kb

4-6 kb

10-12 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following viruses are used as vectors for transferring genes to animal cells?

Retroviruses

Bovine papilloma virus (BVP)

SV 40 virus

All of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Cos cell line is

derivative of non-permissive CV-1 monkey cell line

derivative of permissive CV-1 monkey cell line

cohesive initial site of phage λ

cohesive end site of phage λ

TRUE ANSWER : ?
YOUR ANSWER : ?

The size of the genome of SV 40 is

7.2 kb

110 kb

69 kb

5.2 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

Cloning genes in the yeast offer which of following advantage(s)?

All of these

Ability to glycosylate proteins during secretion

Ability to clone large piece of DNA

Over production of proteins of commercial value

TRUE ANSWER : ?
YOUR ANSWER : ?

Polyhedrin protein is

major component of protein matrix in which viruses are embedded

non essential for baculo virus replication

both (a) and (b)

essential for baculo virus replication

TRUE ANSWER : ?
YOUR ANSWER : ?

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