VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Yeast episomal plasmids (YEp) were constructed using

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

12μm yeast plasmid and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

12μm yeast plasmid and E coli vector pBR 322

TRUE ANSWER : ?
YOUR ANSWER : ?

Autonomously replicating sequence is not present in

YCp

YIp

YRp

YAC

TRUE ANSWER : ?
YOUR ANSWER : ?

Cloning genes in the yeast offer which of following advantage(s)?

Ability to glycosylate proteins during secretion

All of these

Ability to clone large piece of DNA

Over production of proteins of commercial value

TRUE ANSWER : ?
YOUR ANSWER : ?

Recombinant SV40 viruses introduce the foreign DNA into cells

without the RNA mediated transfection

with the RNA mediated transfection

with the DNA mediated transfection

without the DNA mediated transfection

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following histone is not present in SV40 virus?

H1

H3

H4

H2A

TRUE ANSWER : ?
YOUR ANSWER : ?

Polyhedrin protein is

essential for baculo virus replication

non essential for baculo virus replication

major component of protein matrix in which viruses are embedded

both (a) and (b)

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following are produced in high copy number?

YRp

YEp

YCp

Both (a) and (b)

TRUE ANSWER : ?
YOUR ANSWER : ?

SV 40 vectors, in which late region replacements are done, require

pBR 322 vector

none of these

SV 40 helper virus

COS cell line

TRUE ANSWER : ?
YOUR ANSWER : ?

SV 40 vectors, in which early region replacements are carried out, require

none of these

COS cell line

SV 40 helper virus

pBR 322 vector

TRUE ANSWER : ?
YOUR ANSWER : ?

Moloney-Mu-leukemia virus is an example of

Baculo virus

Vaccinia virus

none of these

Retro virus

TRUE ANSWER : ?
YOUR ANSWER : ?

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