VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1

Ura 3

His 3

Lys 2

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following promoters is repressed in presence of glucose?

Both (a) and (b)

Phosphoglycerate kinase (PGK)

Acid phosphatase (PHOS)

Galactokinase (GAL 1)

TRUE ANSWER : ?
YOUR ANSWER : ?

Cloning genes in the yeast offer which of following advantage(s)?

All of these

Over production of proteins of commercial value

Ability to glycosylate proteins during secretion

Ability to clone large piece of DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Papilloma virus

Baculo virus

Retrovirus

Vaccinia virus

TRUE ANSWER : ?
YOUR ANSWER : ?

Yeast Replicating plasmids (YRp) carry

autonomously replicating sequence

centromere

telomere

both (a) and (c)

TRUE ANSWER : ?
YOUR ANSWER : ?

α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1

Lys 2

His 3

Ura 3

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following can be used for reducing the size of gene inserts containing introns?

Baculo virus

none of these

Vaccinia virus

Retro virus

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following viruses are used as vectors for transferring genes to animal cells?

Bovine papilloma virus (BVP)

All of these

SV 40 virus

Retroviruses

TRUE ANSWER : ?
YOUR ANSWER : ?

Which one of the following is the most stable vector?

YIp

YRp

YAC

YEp

TRUE ANSWER : ?
YOUR ANSWER : ?

The size of the genome of SV 40 is

7.2 kb

69 kb

110 kb

5.2 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

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