VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Yeast Replicating plasmids (YRp) carry

both (a) and (c)

centromere

autonomously replicating sequence

telomere

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following are produced in high copy number?

YRp

YEp

YCp

Both (a) and (b)

TRUE ANSWER : ?
YOUR ANSWER : ?

Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

transcriptional regulators and are essential for viral replication

coding for virus coat protein

important for packaging of viral DNA into viral head

not necessary for viral replication

TRUE ANSWER : ?
YOUR ANSWER : ?

Lytic infection of SV 40 does not occur in

both (a) and (b)

non permissive cells

none of these

permissive cells

TRUE ANSWER : ?
YOUR ANSWER : ?

Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

All of these

Contains centromeric sequences

Functions as true chromosome

Segregates during meiosis and mitosis

TRUE ANSWER : ?
YOUR ANSWER : ?

Moloney-Mu-leukemia virus is an example of

Retro virus

none of these

Baculo virus

Vaccinia virus

TRUE ANSWER : ?
YOUR ANSWER : ?

Maximum insert size in case of adenovirus vectors is

6-8 kb

10-12 kb

4-6 kb

20 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

YAC vectors can be used to obtain artificial chromosomes because it carries

telomere

all of these

autonomously replicating sequence

centromere

TRUE ANSWER : ?
YOUR ANSWER : ?

Maximum amount of foreign DNA introduced into SV 40 viruses is

5 kb

10 kb

2.5 kb

1 kb

TRUE ANSWER : ?
YOUR ANSWER : ?

Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries centromere and telomere sequences

Carries autonomously replicating sequence

Allows transformation by using crossing over

Has a origin of replication and rep genes

TRUE ANSWER : ?
YOUR ANSWER : ?

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