VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Ability to clone large piece of DNA

Over production of proteins of commercial value

All of these

Ability to glycosylate proteins during secretion

YIp

YEp

YAC

YRp

both (a) and (c)

telomere

centromere

autonomously replicating sequence

All of these

they cover a wide host range

infection does not lead to cell death, infected cells produce virus over an indefinite period

viral gene expression is driven by strong promoters

non permissive cells

none of these

both (a) and (b)

permissive cells

10-12 kb

20 kb

6-8 kb

4-6 kb

Alcohol dehydrogenase II (ADH 2)

All of these

Phosphoglycerate kinase (PGK)

Galactokinase (GAL 1)

12μm yeast plasmid and E coli vector pMB 9

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

12μm yeast plasmid and E coli vector pBR 322

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

non essential for baculo virus replication

both (a) and (b)

essential for baculo virus replication

major component of protein matrix in which viruses are embedded

integrated only

episomally only

either episomally or integrated at high copy number

either episomally or integrated at low copy number