VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Retroviruses consist of

RNA genome

Ss DNA genome

Ds DNA genome

cDNA genome

TRUE ANSWER : ?
YOUR ANSWER : ?

YAC vectors can be used to obtain artificial chromosomes because it carries

autonomously replicating sequence

telomere

all of these

centromere

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following(s) is/are the most commonly used markers in yeast?

Trp 1

Ura 3

His 3

All of these

TRUE ANSWER : ?
YOUR ANSWER : ?

In case of Baculoviruses, the foreign gene

is inserted downstream of viral origin of replication

is inserted between the polyhedrin promoter and downstream sequences

is inserted downstream of polyhedrin promoter

is inserted randomly in viral genome

TRUE ANSWER : ?
YOUR ANSWER : ?

α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

His 3

Ura 3

Lys 2

Trp 1

TRUE ANSWER : ?
YOUR ANSWER : ?

Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Contains centromeric sequences

Functions as true chromosome

Segregates during meiosis and mitosis

All of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Recombinant SV40 viruses introduce the foreign DNA into cells

with the DNA mediated transfection

with the RNA mediated transfection

without the RNA mediated transfection

without the DNA mediated transfection

TRUE ANSWER : ?
YOUR ANSWER : ?

Which one of the following is the most stable vector?

YEp

YAC

YIp

YRp

TRUE ANSWER : ?
YOUR ANSWER : ?

5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1

His 3

Lys 2

Ura 3

TRUE ANSWER : ?
YOUR ANSWER : ?

Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

transcriptional regulators and are essential for viral replication

coding for virus coat protein

not necessary for viral replication

important for packaging of viral DNA into viral head

TRUE ANSWER : ?
YOUR ANSWER : ?