RECOMBINANT DNA QUIZ

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

Restriction endonuclease

DNA helicase

DNA polymerase

DNA ligase

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Which of the following vector can maintain the largest fragment of foreign DNA?

Phage

Plasmid

YAC

Cosmid

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The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

DNA probe

inoculating needle

laser

gene gun

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In genetic engineering, a chimera is

a virus that infects bacteria

a plasmid that contains foreign DNA

a fungi

an enzyme that links DNA molecules

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An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

a vector

a transgenic animal

an enzyme that links DNA molecules

a chimera

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What is the normal role of restriction endonucleases in bacterial cells?

All of these

To produce RNA primers for replication

To degrade the bacterial chromosome into small pieces during replication

To degrade invading phage DNA

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Which of the following is obtained using processed mRNA molecules as a template?

mDNA

rDNA

tDNA

cDNA

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A genomic library is

a book that describes how to isolate DNA from a particular organism

a database where the sequence of an organism's genome is stored

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

a place where the information of the genetic organization of organisms are kept.

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

microbiology

genetic engineering

population genetics

protein engineering

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The Southern blotting technique depends on

the molecular mass of proteins

similarities between the sequences of probe RNA and experimental RNA

similarities between the sequences of probe DNA and experimental DNA

similarities between the sequences of probe protein and experimental protein

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