RECOMBINANT DNA QUIZ

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

DNA polymerase

DNA helicase

DNA ligase

Restriction endonuclease

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Electroporation is

the process of combining foreign DNA to an electrically charged vector molecule

the process of multiplication of the cells

the process of separating charged molecules through a gel maintained in an electric field

the application of high voltage pulses

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

DNA replication

polymerase chain reaction

southern blotting

northern blotting

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Which of the following vector can maintain the largest fragment of foreign DNA?

Cosmid

YAC

Phage

Plasmid

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

All of these

the DNA polymerases of these bacteria are much faster than those from other organisms

the DNA polymerases of these bacteria never make mistakes while replicating DNA

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

helicases

DNA gyrase

restriction endonucleases

DNA ligase

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

microbiology

population genetics

genetic engineering

protein engineering

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Which type of restriction endonuclease cuts the DNA within the recognition site?

All of the these

Type III

Type II

Type I

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Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

gel electrophoresis

autoradiography

photoreactivation

blotting

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Bacterial cells protect their own DNA from degradation by restriction endonucleases by

deleting all recognition sites from the genome

having anti restriction endonucleases

not producing any restriction endonucleases

methylating the DNA at the sites that the enzyme recognizes

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