RECOMBINANT DNA QUIZ

For gene probes to be useful they must

both (a) and (b)

be large enough to contain gene-specific sequences

None of these

be labeled in some manner to allow detection

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Agrobacterium tumefaciens is

a bacterium that can be used to introduce DNA into plants

a disease in humans that causes loss of weight

a disease in humans that causes loss of sight

a fungi that is used to produce antibiotics in large amounts

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Which of the following is obtained using processed mRNA molecules as a template?

cDNA

tDNA

mDNA

rDNA

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

the DNA polymerases of these bacteria are much faster than those from other organisms

All of these

the DNA polymerases of these bacteria never make mistakes while replicating DNA

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

protein engineering

microbiology

genetic engineering

population genetics

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In genetic engineering, a chimera is

a virus that infects bacteria

a plasmid that contains foreign DNA

an enzyme that links DNA molecules

a fungi

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Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

YACS

BACS

all of these

expression vectors

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Electroporation is

the process of combining foreign DNA to an electrically charged vector molecule

the process of separating charged molecules through a gel maintained in an electric field

the application of high voltage pulses

the process of multiplication of the cells

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

helicases

DNA ligase

restriction endonucleases

DNA gyrase

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Which of the following vector can maintain the largest fragment of foreign DNA?

Phage

Cosmid

YAC

Plasmid

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