RECOMBINANT DNA QUIZ

Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

gel electrophoresis

autoradiography

blotting

photoreactivation

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The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

inoculating needle

gene gun

DNA probe

laser

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Agrobacterium tumefaciens is

a fungi that is used to produce antibiotics in large amounts

a disease in humans that causes loss of weight

a disease in humans that causes loss of sight

a bacterium that can be used to introduce DNA into plants

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

northern blotting

southern blotting

DNA replication

polymerase chain reaction

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

protein engineering

microbiology

genetic engineering

population genetics

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The Southern blotting technique depends on

similarities between the sequences of probe protein and experimental protein

the molecular mass of proteins

similarities between the sequences of probe RNA and experimental RNA

similarities between the sequences of probe DNA and experimental DNA

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Electroporation is

the process of multiplication of the cells

the process of separating charged molecules through a gel maintained in an electric field

the process of combining foreign DNA to an electrically charged vector molecule

the application of high voltage pulses

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria are much faster than those from other organisms

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

All of these

the DNA polymerases of these bacteria never make mistakes while replicating DNA

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Which of the following is not commonly used as vector?

Cosmid

Fungi

Plasmid

Artificial chromosome

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What is the normal role of restriction endonucleases in bacterial cells?

To degrade invading phage DNA

All of these

To produce RNA primers for replication

To degrade the bacterial chromosome into small pieces during replication

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