RECOMBINANT DNA QUIZ

Electroporation is

the application of high voltage pulses

the process of multiplication of the cells

the process of separating charged molecules through a gel maintained in an electric field

the process of combining foreign DNA to an electrically charged vector molecule

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Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

all of these

BACS

YACS

expression vectors

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

All of these

the DNA polymerases of these bacteria never make mistakes while replicating DNA

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

the DNA polymerases of these bacteria are much faster than those from other organisms

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

southern blotting

northern blotting

DNA replication

polymerase chain reaction

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Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

photoreactivation

gel electrophoresis

blotting

autoradiography

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Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

DNA polymerase

Restriction endonuclease

DNA ligase

DNA helicase

TRUE ANSWER : ?
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A short molecule containing 2-20 nucleotide is

plasmid

vector

oligonucleotide

mononucleotide

TRUE ANSWER : ?
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What is the normal role of restriction endonucleases in bacterial cells?

To degrade the bacterial chromosome into small pieces during replication

To degrade invading phage DNA

To produce RNA primers for replication

All of these

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For gene probes to be useful they must

be large enough to contain gene-specific sequences

both (a) and (b)

None of these

be labeled in some manner to allow detection

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Which of the following is obtained using processed mRNA molecules as a template?

rDNA

tDNA

cDNA

mDNA

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