RECOMBINANT DNA QUIZ

For gene probes to be useful they must

None of these

be labeled in some manner to allow detection

both (a) and (b)

be large enough to contain gene-specific sequences

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Which of the following is not commonly used as vector?

Fungi

Artificial chromosome

Cosmid

Plasmid

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Which of the following vector can maintain the largest fragment of foreign DNA?

Plasmid

Phage

Cosmid

YAC

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Electroporation is

the process of combining foreign DNA to an electrically charged vector molecule

the process of separating charged molecules through a gel maintained in an electric field

the process of multiplication of the cells

the application of high voltage pulses

TRUE ANSWER : ?
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Agrobacterium tumefaciens is

a bacterium that can be used to introduce DNA into plants

a disease in humans that causes loss of weight

a disease in humans that causes loss of sight

a fungi that is used to produce antibiotics in large amounts

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In genetic engineering, a chimera is

an enzyme that links DNA molecules

a virus that infects bacteria

a plasmid that contains foreign DNA

a fungi

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An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

a chimera

an enzyme that links DNA molecules

a vector

a transgenic animal

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Bacterial cells protect their own DNA from degradation by restriction endonucleases by

having anti restriction endonucleases

methylating the DNA at the sites that the enzyme recognizes

deleting all recognition sites from the genome

not producing any restriction endonucleases

TRUE ANSWER : ?
YOUR ANSWER : ?

The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

microbiology

genetic engineering

protein engineering

population genetics

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Which type of restriction endonuclease cuts the DNA within the recognition site?

All of the these

Type II

Type I

Type III

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