RECOMBINANT DNA QUIZ

What is the normal role of restriction endonucleases in bacterial cells?

To produce RNA primers for replication

To degrade invading phage DNA

To degrade the bacterial chromosome into small pieces during replication

All of these

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

polymerase chain reaction

southern blotting

northern blotting

DNA replication

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Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

photoreactivation

autoradiography

gel electrophoresis

blotting

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For gene probes to be useful they must

both (a) and (b)

be labeled in some manner to allow detection

None of these

be large enough to contain gene-specific sequences

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The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

gene gun

laser

DNA probe

inoculating needle

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Which of the following is not commonly used as vector?

Fungi

Cosmid

Artificial chromosome

Plasmid

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A genomic library is

a database where the sequence of an organism's genome is stored

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

a book that describes how to isolate DNA from a particular organism

a place where the information of the genetic organization of organisms are kept.

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

restriction endonucleases

DNA gyrase

DNA ligase

helicases

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Which of the following is obtained using processed mRNA molecules as a template?

mDNA

rDNA

cDNA

tDNA

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Electroporation is

the process of combining foreign DNA to an electrically charged vector molecule

the application of high voltage pulses

the process of separating charged molecules through a gel maintained in an electric field

the process of multiplication of the cells

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