RECOMBINANT DNA QUIZ

Which of the following is not commonly used as vector?

Cosmid

Fungi

Artificial chromosome

Plasmid

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria never make mistakes while replicating DNA

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

the DNA polymerases of these bacteria are much faster than those from other organisms

All of these

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Which of the following vector can maintain the largest fragment of foreign DNA?

Phage

YAC

Plasmid

Cosmid

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Agrobacterium tumefaciens is

a disease in humans that causes loss of weight

a disease in humans that causes loss of sight

a fungi that is used to produce antibiotics in large amounts

a bacterium that can be used to introduce DNA into plants

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The Southern blotting technique depends on

similarities between the sequences of probe RNA and experimental RNA

similarities between the sequences of probe DNA and experimental DNA

similarities between the sequences of probe protein and experimental protein

the molecular mass of proteins

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Which of the following is obtained using processed mRNA molecules as a template?

cDNA

mDNA

tDNA

rDNA

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For gene probes to be useful they must

None of these

be labeled in some manner to allow detection

both (a) and (b)

be large enough to contain gene-specific sequences

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Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

photoreactivation

gel electrophoresis

blotting

autoradiography

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Vectors are

molecules that help in replication

molecules that degrade nucleic acids

molecules that protect host cells from invasion by foreign DNA

molecules that are able to covalently bond to and carry foreign DNA into cells

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What is the normal role of restriction endonucleases in bacterial cells?

To produce RNA primers for replication

To degrade the bacterial chromosome into small pieces during replication

All of these

To degrade invading phage DNA

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