RECOMBINANT DNA QUIZ

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

polymerase chain reaction

northern blotting

DNA replication

southern blotting

TRUE ANSWER : ?
YOUR ANSWER : ?

A short molecule containing 2-20 nucleotide is

mononucleotide

plasmid

vector

oligonucleotide

TRUE ANSWER : ?
YOUR ANSWER : ?

Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

all of these

BACS

YACS

expression vectors

TRUE ANSWER : ?
YOUR ANSWER : ?

Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

photoreactivation

blotting

autoradiography

gel electrophoresis

TRUE ANSWER : ?
YOUR ANSWER : ?

Which type of restriction endonuclease cuts the DNA within the recognition site?

Type II

Type I

Type III

All of the these

TRUE ANSWER : ?
YOUR ANSWER : ?

What is the normal role of restriction endonucleases in bacterial cells?

To degrade the bacterial chromosome into small pieces during replication

To degrade invading phage DNA

To produce RNA primers for replication

All of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Bacterial cells protect their own DNA from degradation by restriction endonucleases by

methylating the DNA at the sites that the enzyme recognizes

deleting all recognition sites from the genome

having anti restriction endonucleases

not producing any restriction endonucleases

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is obtained using processed mRNA molecules as a template?

tDNA

rDNA

cDNA

mDNA

TRUE ANSWER : ?
YOUR ANSWER : ?

Electroporation is

the application of high voltage pulses

the process of separating charged molecules through a gel maintained in an electric field

the process of multiplication of the cells

the process of combining foreign DNA to an electrically charged vector molecule

TRUE ANSWER : ?
YOUR ANSWER : ?

An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

a chimera

an enzyme that links DNA molecules

a vector

a transgenic animal

TRUE ANSWER : ?
YOUR ANSWER : ?