RECOMBINANT DNA QUIZ

Electroporation is

the process of separating charged molecules through a gel maintained in an electric field

the process of multiplication of the cells

the application of high voltage pulses

the process of combining foreign DNA to an electrically charged vector molecule

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What is the normal role of restriction endonucleases in bacterial cells?

To produce RNA primers for replication

All of these

To degrade the bacterial chromosome into small pieces during replication

To degrade invading phage DNA

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Which type of restriction endonuclease cuts the DNA within the recognition site?

All of the these

Type III

Type I

Type II

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

DNA ligase

helicases

restriction endonucleases

DNA gyrase

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Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

DNA helicase

DNA polymerase

Restriction endonuclease

DNA ligase

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

DNA replication

northern blotting

polymerase chain reaction

southern blotting

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For gene probes to be useful they must

be labeled in some manner to allow detection

both (a) and (b)

None of these

be large enough to contain gene-specific sequences

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Agrobacterium tumefaciens is

a bacterium that can be used to introduce DNA into plants

a fungi that is used to produce antibiotics in large amounts

a disease in humans that causes loss of sight

a disease in humans that causes loss of weight

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

microbiology

protein engineering

population genetics

genetic engineering

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The Southern blotting technique depends on

the molecular mass of proteins

similarities between the sequences of probe protein and experimental protein

similarities between the sequences of probe RNA and experimental RNA

similarities between the sequences of probe DNA and experimental DNA

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