RECOMBINANT DNA QUIZ

The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

protein engineering

genetic engineering

population genetics

microbiology

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The Southern blotting technique depends on

similarities between the sequences of probe DNA and experimental DNA

similarities between the sequences of probe RNA and experimental RNA

the molecular mass of proteins

similarities between the sequences of probe protein and experimental protein

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Which type of restriction endonuclease cuts the DNA within the recognition site?

Type III

Type II

All of the these

Type I

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

southern blotting

polymerase chain reaction

northern blotting

DNA replication

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What is the normal role of restriction endonucleases in bacterial cells?

To degrade the bacterial chromosome into small pieces during replication

To produce RNA primers for replication

All of these

To degrade invading phage DNA

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

All of these

the DNA polymerases of these bacteria are much faster than those from other organisms

the DNA polymerases of these bacteria never make mistakes while replicating DNA

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An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

a vector

a chimera

an enzyme that links DNA molecules

a transgenic animal

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Vectors are

molecules that degrade nucleic acids

molecules that protect host cells from invasion by foreign DNA

molecules that are able to covalently bond to and carry foreign DNA into cells

molecules that help in replication

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

restriction endonucleases

helicases

DNA ligase

DNA gyrase

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Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

Restriction endonuclease

DNA ligase

DNA helicase

DNA polymerase

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