RECOMBINANT DNA QUIZ

Bacterial cells protect their own DNA from degradation by restriction endonucleases by

methylating the DNA at the sites that the enzyme recognizes

having anti restriction endonucleases

deleting all recognition sites from the genome

not producing any restriction endonucleases

TRUE ANSWER : ?
YOUR ANSWER : ?

Electroporation is

the process of combining foreign DNA to an electrically charged vector molecule

the application of high voltage pulses

the process of multiplication of the cells

the process of separating charged molecules through a gel maintained in an electric field

TRUE ANSWER : ?
YOUR ANSWER : ?

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

polymerase chain reaction

northern blotting

southern blotting

DNA replication

TRUE ANSWER : ?
YOUR ANSWER : ?

An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

a transgenic animal

a chimera

an enzyme that links DNA molecules

a vector

TRUE ANSWER : ?
YOUR ANSWER : ?

The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

All of these

the DNA polymerases of these bacteria are much faster than those from other organisms

the DNA polymerases of these bacteria never make mistakes while replicating DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

In genetic engineering, a chimera is

a fungi

an enzyme that links DNA molecules

a virus that infects bacteria

a plasmid that contains foreign DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

A genomic library is

a place where the information of the genetic organization of organisms are kept.

a book that describes how to isolate DNA from a particular organism

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

a database where the sequence of an organism's genome is stored

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following vector can maintain the largest fragment of foreign DNA?

Phage

Cosmid

YAC

Plasmid

TRUE ANSWER : ?
YOUR ANSWER : ?

A short molecule containing 2-20 nucleotide is

oligonucleotide

vector

mononucleotide

plasmid

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is not commonly used as vector?

Fungi

Artificial chromosome

Cosmid

Plasmid

TRUE ANSWER : ?
YOUR ANSWER : ?