RECOMBINANT DNA QUIZ

Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

blotting

autoradiography

gel electrophoresis

photoreactivation

TRUE ANSWER : ?
YOUR ANSWER : ?

Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

helicases

restriction endonucleases

DNA ligase

DNA gyrase

TRUE ANSWER : ?
YOUR ANSWER : ?

An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

a chimera

an enzyme that links DNA molecules

a transgenic animal

a vector

TRUE ANSWER : ?
YOUR ANSWER : ?

Which type of restriction endonuclease cuts the DNA within the recognition site?

Type II

Type III

All of the these

Type I

TRUE ANSWER : ?
YOUR ANSWER : ?

Vectors are

molecules that are able to covalently bond to and carry foreign DNA into cells

molecules that help in replication

molecules that degrade nucleic acids

molecules that protect host cells from invasion by foreign DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

A short molecule containing 2-20 nucleotide is

oligonucleotide

mononucleotide

vector

plasmid

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

Restriction endonuclease

DNA ligase

DNA helicase

DNA polymerase

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is not commonly used as vector?

Fungi

Cosmid

Plasmid

Artificial chromosome

TRUE ANSWER : ?
YOUR ANSWER : ?

What is the normal role of restriction endonucleases in bacterial cells?

To produce RNA primers for replication

To degrade the bacterial chromosome into small pieces during replication

All of these

To degrade invading phage DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

southern blotting

polymerase chain reaction

DNA replication

northern blotting

TRUE ANSWER : ?
YOUR ANSWER : ?