RECOMBINANT DNA QUIZ

Which type of restriction endonuclease cuts the DNA within the recognition site?

Type I

Type II

Type III

All of the these

TRUE ANSWER : ?
YOUR ANSWER : ?

Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

BACS

YACS

expression vectors

all of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is not commonly used as vector?

Cosmid

Fungi

Plasmid

Artificial chromosome

TRUE ANSWER : ?
YOUR ANSWER : ?

The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

population genetics

genetic engineering

protein engineering

microbiology

TRUE ANSWER : ?
YOUR ANSWER : ?

For gene probes to be useful they must

be labeled in some manner to allow detection

both (a) and (b)

be large enough to contain gene-specific sequences

None of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following vector can maintain the largest fragment of foreign DNA?

Cosmid

Plasmid

YAC

Phage

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is obtained using processed mRNA molecules as a template?

tDNA

mDNA

rDNA

cDNA

TRUE ANSWER : ?
YOUR ANSWER : ?

Vectors are

molecules that protect host cells from invasion by foreign DNA

molecules that are able to covalently bond to and carry foreign DNA into cells

molecules that help in replication

molecules that degrade nucleic acids

TRUE ANSWER : ?
YOUR ANSWER : ?

Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

gel electrophoresis

blotting

photoreactivation

autoradiography

TRUE ANSWER : ?
YOUR ANSWER : ?

Electroporation is

the process of separating charged molecules through a gel maintained in an electric field

the application of high voltage pulses

the process of combining foreign DNA to an electrically charged vector molecule

the process of multiplication of the cells

TRUE ANSWER : ?
YOUR ANSWER : ?