RECOMBINANT DNA QUIZ

In genetic engineering, a chimera is

an enzyme that links DNA molecules

a fungi

a virus that infects bacteria

a plasmid that contains foreign DNA

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The Southern blotting technique depends on

the molecular mass of proteins

similarities between the sequences of probe RNA and experimental RNA

similarities between the sequences of probe DNA and experimental DNA

similarities between the sequences of probe protein and experimental protein

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Which type of restriction endonuclease cuts the DNA within the recognition site?

All of the these

Type II

Type III

Type I

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Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

gel electrophoresis

photoreactivation

blotting

autoradiography

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The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

gene gun

DNA probe

inoculating needle

laser

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria are much faster than those from other organisms

All of these

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

the DNA polymerases of these bacteria never make mistakes while replicating DNA

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Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

all of these

expression vectors

YACS

BACS

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Which of the following is obtained using processed mRNA molecules as a template?

tDNA

rDNA

cDNA

mDNA

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A genomic library is

a database where the sequence of an organism's genome is stored

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

a place where the information of the genetic organization of organisms are kept.

a book that describes how to isolate DNA from a particular organism

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

northern blotting

DNA replication

southern blotting

polymerase chain reaction

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