RECOMBINANT DNA QUIZ

In genetic engineering, a chimera is

a fungi

a virus that infects bacteria

a plasmid that contains foreign DNA

an enzyme that links DNA molecules

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Which of the following is not commonly used as vector?

Artificial chromosome

Plasmid

Fungi

Cosmid

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The Southern blotting technique depends on

similarities between the sequences of probe DNA and experimental DNA

similarities between the sequences of probe RNA and experimental RNA

the molecular mass of proteins

similarities between the sequences of probe protein and experimental protein

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Which of the following is obtained using processed mRNA molecules as a template?

tDNA

rDNA

cDNA

mDNA

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

All of these

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

the DNA polymerases of these bacteria are much faster than those from other organisms

the DNA polymerases of these bacteria never make mistakes while replicating DNA

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

helicases

DNA ligase

restriction endonucleases

DNA gyrase

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A genomic library is

a book that describes how to isolate DNA from a particular organism

a place where the information of the genetic organization of organisms are kept.

a database where the sequence of an organism's genome is stored

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

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The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

DNA probe

gene gun

inoculating needle

laser

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Which of the following vector can maintain the largest fragment of foreign DNA?

YAC

Cosmid

Phage

Plasmid

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Electroporation is

the process of combining foreign DNA to an electrically charged vector molecule

the process of separating charged molecules through a gel maintained in an electric field

the process of multiplication of the cells

the application of high voltage pulses

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