RECOMBINANT DNA QUIZ

The Southern blotting technique depends on

similarities between the sequences of probe protein and experimental protein

the molecular mass of proteins

similarities between the sequences of probe DNA and experimental DNA

similarities between the sequences of probe RNA and experimental RNA

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Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

DNA helicase

DNA polymerase

DNA ligase

Restriction endonuclease

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

population genetics

microbiology

protein engineering

genetic engineering

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Vectors are

molecules that are able to covalently bond to and carry foreign DNA into cells

molecules that help in replication

molecules that degrade nucleic acids

molecules that protect host cells from invasion by foreign DNA

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Which of the following vector can maintain the largest fragment of foreign DNA?

YAC

Cosmid

Plasmid

Phage

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An animal, that has gained new genetic information from the acquisition of foreign DNA, is considered as

a vector

a chimera

a transgenic animal

an enzyme that links DNA molecules

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Bacterial cells protect their own DNA from degradation by restriction endonucleases by

deleting all recognition sites from the genome

methylating the DNA at the sites that the enzyme recognizes

not producing any restriction endonucleases

having anti restriction endonucleases

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

polymerase chain reaction

DNA replication

northern blotting

southern blotting

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Agrobacterium tumefaciens is

a bacterium that can be used to introduce DNA into plants

a disease in humans that causes loss of sight

a disease in humans that causes loss of weight

a fungi that is used to produce antibiotics in large amounts

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What is the normal role of restriction endonucleases in bacterial cells?

To produce RNA primers for replication

To degrade the bacterial chromosome into small pieces during replication

To degrade invading phage DNA

All of these

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