RECOMBINANT DNA QUIZ

Vectors are

molecules that protect host cells from invasion by foreign DNA

molecules that help in replication

molecules that degrade nucleic acids

molecules that are able to covalently bond to and carry foreign DNA into cells

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The piece of equipment, that introduces DNA into cells via DNA-coated microprojectiles is known as

DNA probe

laser

gene gun

inoculating needle

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Charged molecules are separated based on varying rates of migration through a solid matrix when subjected to an electric field. This technique is known as

autoradiography

blotting

photoreactivation

gel electrophoresis

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The deliberate modifications of an organism's genetic information by directly changing its nucleic acid content is a subject matter of

population genetics

microbiology

protein engineering

genetic engineering

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Which of the following is obtained using processed mRNA molecules as a template?

tDNA

mDNA

rDNA

cDNA

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Agrobacterium tumefaciens is

a bacterium that can be used to introduce DNA into plants

a fungi that is used to produce antibiotics in large amounts

a disease in humans that causes loss of sight

a disease in humans that causes loss of weight

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A short molecule containing 2-20 nucleotide is

plasmid

oligonucleotide

mononucleotide

vector

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For gene probes to be useful they must

be labeled in some manner to allow detection

be large enough to contain gene-specific sequences

both (a) and (b)

None of these

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

DNA gyrase

restriction endonucleases

DNA ligase

helicases

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Which of the following is not commonly used as vector?

Fungi

Plasmid

Cosmid

Artificial chromosome

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