PROTEIN AND NUCLEIC ACID INTERACTIONS QUIZ

Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

Leu and Ala

Lys and Arg

Cys and Met

Ile and Val

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YOUR ANSWER : ?

Reverse transcripase from HIV does not

requires a primer

has a 3'- 5' exonuclease activity

has a 5' to 3' polymerase activity

uses RNA as a template

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Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

3' to 5' direction

negative direction

5' to 3' direction

positive direction

TRUE ANSWER : ?
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Cleavage of DNA by EcoRI endonuclease results in the formation of

one 3'-OH end and one 5'-phosphate end

two 3'-OH ends and two 5'-phosphate ends

two 3'-phosphate ends and two 3'-OH ends

one 3'-OH end and two 5'-phosphate end

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

Cys and Met

Asn and Gln

Lys and Leu

Ile and Val

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The type II restriction endonucleases, bind to DNA sites

using non-sequence-dependent backbone interactions

using H-bonds for specificity

that are usually palindromic

All of these

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The enzyme that joins DNA cuts is called

ligase

joinase

DNA phosphorylase

reverse transcriptase

TRUE ANSWER : ?
YOUR ANSWER : ?

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