PROTEIN AND NUCLEIC ACID INTERACTIONS QUIZ

The type II restriction endonucleases, bind to DNA sites

using non-sequence-dependent backbone interactions

that are usually palindromic

All of these

using H-bonds for specificity

TRUE ANSWER : ?
YOUR ANSWER : ?

Cleavage of DNA by EcoRI endonuclease results in the formation of

two 3'-phosphate ends and two 3'-OH ends

one 3'-OH end and one 5'-phosphate end

one 3'-OH end and two 5'-phosphate end

two 3'-OH ends and two 5'-phosphate ends

TRUE ANSWER : ?
YOUR ANSWER : ?

Reverse transcripase from HIV does not

has a 5' to 3' polymerase activity

has a 3'- 5' exonuclease activity

uses RNA as a template

requires a primer

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

Leu and Ala

Ile and Val

Lys and Arg

Cys and Met

TRUE ANSWER : ?
YOUR ANSWER : ?

The enzyme that joins DNA cuts is called

DNA phosphorylase

reverse transcriptase

joinase

ligase

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

Ile and Val

Asn and Gln

Lys and Leu

Cys and Met

TRUE ANSWER : ?
YOUR ANSWER : ?

Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

3' to 5' direction

negative direction

positive direction

5' to 3' direction

TRUE ANSWER : ?
YOUR ANSWER : ?

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