POLYMERASE CHAIN REACTION QUIZ

Taq polymerase

all of these

E. coli DNA polymerase

E. coli RNA polymerase

c DNA

r RNA

m DNA

t RNA

none of these

preventing contamination by outside DNA

the amplification process

separating the different rRNA gene sequences in the mixture

Hepatitis B virus

HIV-1 and HIV-2 viruses

All of these

Mycobacterium tuberculosis

none of these

single stranded DNA

double stranded DNA

RNA

reduction in substrate concentration

build up of PCR product which competes with primers for hybrid formation

insufficient enzyme and time to synthesize mass quantity of DNA

all of these

whether the bacterium is motile

morphology of the bacterium

the species to which the bacteria are most closely related

the type of energy metabolism the bacterium has

three steps

two steps

five steps

four steps