POLYMERASE CHAIN REACTION QUIZ

Mycobacterium tuberculosis

Hepatitis B virus

HIV-1 and HIV-2 viruses

All of these

insufficient enzyme and time to synthesize mass quantity of DNA

reduction in substrate concentration

build up of PCR product which competes with primers for hybrid formation

all of these

single stranded DNA

RNA

double stranded DNA

none of these

whether the bacterium is motile

the species to which the bacteria are most closely related

morphology of the bacterium

the type of energy metabolism the bacterium has

E. coli DNA polymerase

E. coli RNA polymerase

Taq polymerase

all of these

two steps

three steps

five steps

four steps

c DNA

m DNA

r RNA

t RNA

separating the different rRNA gene sequences in the mixture

preventing contamination by outside DNA

none of these

the amplification process