POLYMERASE CHAIN REACTION QUIZ

RNA

single stranded DNA

double stranded DNA

none of these

the type of energy metabolism the bacterium has

whether the bacterium is motile

morphology of the bacterium

the species to which the bacteria are most closely related

All of these

HIV-1 and HIV-2 viruses

Mycobacterium tuberculosis

Hepatitis B virus

the amplification process

none of these

separating the different rRNA gene sequences in the mixture

preventing contamination by outside DNA

m DNA

c DNA

r RNA

t RNA

E. coli RNA polymerase

E. coli DNA polymerase

Taq polymerase

all of these

reduction in substrate concentration

build up of PCR product which competes with primers for hybrid formation

all of these

insufficient enzyme and time to synthesize mass quantity of DNA

two steps

five steps

four steps

three steps