POLYMERASE CHAIN REACTION QUIZ

insufficient enzyme and time to synthesize mass quantity of DNA

build up of PCR product which competes with primers for hybrid formation

all of these

reduction in substrate concentration

four steps

two steps

five steps

three steps

whether the bacterium is motile

the type of energy metabolism the bacterium has

morphology of the bacterium

the species to which the bacteria are most closely related

All of these

Mycobacterium tuberculosis

Hepatitis B virus

HIV-1 and HIV-2 viruses

m DNA

t RNA

c DNA

r RNA

Taq polymerase

E. coli RNA polymerase

E. coli DNA polymerase

all of these

single stranded DNA

double stranded DNA

none of these

RNA

separating the different rRNA gene sequences in the mixture

preventing contamination by outside DNA

the amplification process

none of these