The cloning step in PCR/sequencing analysis of microbial communities is necessary for separating the different rRNA gene sequences in the mixture none of these preventing contamination by outside DNA the amplification process TRUE ANSWER : ? YOUR ANSWER : ?
The efficiency of amplification in PCR in later cycles is reduced due to insufficient enzyme and time to synthesize mass quantity of DNA reduction in substrate concentration all of these build up of PCR product which competes with primers for hybrid formation TRUE ANSWER : ? YOUR ANSWER : ?
RNA populations can also be used as PCR templates after reverse transcription into c DNA r RNA m DNA t RNA TRUE ANSWER : ? YOUR ANSWER : ?
The PCR/sequencing approach to rRNA is most likely to give information about whether the bacterium is motile the species to which the bacteria are most closely related the type of energy metabolism the bacterium has morphology of the bacterium TRUE ANSWER : ? YOUR ANSWER : ?
Polymerase chain reaction basically consists of five steps two steps three steps four steps TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction? HIV-1 and HIV-2 viruses All of these Mycobacterium tuberculosis Hepatitis B virus TRUE ANSWER : ? YOUR ANSWER : ?
The original enzyme(s) used in PCR reaction is/are known as E. coli DNA polymerase all of these Taq polymerase E. coli RNA polymerase TRUE ANSWER : ? YOUR ANSWER : ?
The template for PCR is RNA single stranded DNA double stranded DNA none of these TRUE ANSWER : ? YOUR ANSWER : ?