NUCLEIC ACIDS QUIZ

Nucleotide bases and aromatic amino acids absorb light respectively at

280 and 260 nm

270 and 280 nm

260 and 270 nm

260 and 280 nm

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Which of the following is found on RNA but not DNA?

Uracil

Deoxyribose

Adenine

Phosphate

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In gel electrophoresis, what fragments will move most quickly through a gel?

Large genome

Small fragments

None of these

Large fragments

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The glycosidic bonds in DNA and RNA

stabilize Watson-Crick H-bonds

can be hydrolyzed by OH- ion

connect the sugar to the base

are free to rotate over about 180°

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The unfavourable free energy associated with not satisfying a hydrogen bond after formation of the double helix is approximately

10 kJ/mol

25 kJ/mol

20 kJ/mol

30 kJ/mol

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Thymidine

normally forms two hydrogen bonds with adenosine

can participate in hydrophobic interactions due to its methyl group

All of these

is replaced by uracil in RNA

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The most stabilizing force for nucleic acids is

electrostatic bond

conformational entropy

Van der Waals

hydrogen bonds

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A five carbon sugar lacking a hydrogen at the number 2 carbon is found in

mRNA

DNA

RNA

rDNA

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Which pyrimidine base contains an amino group at carbon 4?

Adenine

Cytosine

Uracil

Thymine

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A nucleotide consists of

a sugar, a base and a phosphate

a sugar and a phosphate

a sugar, a base and three phosphates

paired bases

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