MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Which type of plasmid can exist with or without being integrated into the host's chromosome?

Lisosome

Lysogen

Episome

Medisome

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When composite transposons are formed

two IS elements integrate into a chromosome with only a small distance separating them

a small deletion occurs in the transposase gene of an IS element

a small deletion occurs in the transposase gene of an IS element and plasmid is integrated

an IS element integrates with another IS element with the help of a plasmid

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The plasmids can be eliminated from a cell by the process known as

breaking

curing

fixing

expulsion

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Who discovered transposons (jumping genes)?

Griffith

Harvey

McClintock

Abelson

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The transducing particles carry only specific portions of the bacterial genome in which of the following transduction?

None of these

Specialized transduction

Abortive transduction

General transduction

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The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

transfers both strands of the plasmid DNA

usually has a transposon inserted into it

carries genes encoding the mating apparatus

transfers antibiotic resistance genes

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The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

allow to monitor the expression of all genes with a promoter similar in sequence to Px

have the promoter of lacZ or some other reporter gene

give the same information as from a microarray

have Px but not the rest of the X coding region

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Recombination of virus genomes occurs

by transformation

simultaneous infection of a host cell by two viruses with homologous chromosomes

by transduction

by transription

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Which of the following plamids do not possess information for self transfer to another cell?

Conjugative plasmids

None of these

Cryptic plasmids

Non-conjugative plasmids

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In lysogeny,

bacteria take up double stranded DNA from the environment

DNA-degrading enzymes in the extracellular medium would stop the process

a bacteriophage transfers bacterial DNA

a bacteriophage genome is integrated into the bacterial genome

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