MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Which of the following is used by microbial genetisists as a tool?

Transposable elements

All of these

Plasmids

Bacteriophage

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Recombination of virus genomes occurs

by transformation

by transription

simultaneous infection of a host cell by two viruses with homologous chromosomes

by transduction

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What information can be generated by interrupted mating experiments?

DNA nucleotide sequences

Bacterial genome maps

Proteomics of the bacteria

Levels of DNA homology

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Which of the following is the cause for drug resistance in tuberculosis?

Conjugation

Transduction

Mutation

Transformation

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Which of the following type of recombination does not require homologous sequences and is utilized by mobile genetic elements that move about chromosomes?

Site-specific recombination

General recombination

Mutagenic recombimation

Replicative recombination

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The plasmid which makes the host more pathogenic is

F factors

Metabolic plasmid

None of these

Virulence plasmid

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Which of the following statement can describe horizontal transfer?

The synthesis of RNA from a DNA template

The transmission of genetic information from parent to offspring

The transmission of genetic information from one independent, mature organism to another

The synthesis of protein in RNA

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Which of the following term describes the relationship between a virus and host where no new viral particles are produced and the viral genome is replicated along with host chromosome?

Lysogeny

Conjugation

Lysis

Transformation

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The correct term for the transfer of genetic material between bacteria in direct physical contact is

replication

conjugation

transduction

transformation

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The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

give the same information as from a microarray

have the promoter of lacZ or some other reporter gene

have Px but not the rest of the X coding region

allow to monitor the expression of all genes with a promoter similar in sequence to Px

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