MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Recombination of virus genomes occurs

by transription

by transformation

simultaneous infection of a host cell by two viruses with homologous chromosomes

by transduction

TRUE ANSWER : ?
YOUR ANSWER : ?

The chromosomal genes, possessing fertility factor is known as

F factor

HFr

R factor

F prime factor

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is used by microbial genetisists as a tool?

Bacteriophage

All of these

Transposable elements

Plasmids

TRUE ANSWER : ?
YOUR ANSWER : ?

The plasmid which makes the host more pathogenic is

Virulence plasmid

F factors

None of these

Metabolic plasmid

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following statement(s) is/are true in regards to F+ x F- mating events?

No DNA is transferred because F+ cells are unable to perform conjugation

DNA is transferred from F- to F+ cells

No DNA is transferred because F- cells are unable to perform conjugation

DNA is transferred from F+ to F- cells

TRUE ANSWER : ?
YOUR ANSWER : ?

R factors involved in plant-microbe interactions are

essential for transfer of DNA to plant cells

also called opines

bacterial proteins

plant proteins

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

General recombination

Site-specific recombination

Replicative recombination

Mutagenic recombimation

TRUE ANSWER : ?
YOUR ANSWER : ?

F factor plasmids play a major role in

transduction

conjugation

trasnscription

replication

TRUE ANSWER : ?
YOUR ANSWER : ?

The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

have Px but not the rest of the X coding region

have the promoter of lacZ or some other reporter gene

allow to monitor the expression of all genes with a promoter similar in sequence to Px

give the same information as from a microarray

TRUE ANSWER : ?
YOUR ANSWER : ?

A microarray differs from a gene fusion in that, it

gives direct measurement of mRNA level

All of these

is not constructed by cloning

carries DNA segments from many different genes

TRUE ANSWER : ?
YOUR ANSWER : ?