MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Penicillin resistance in staphylococci is acquired due to

transduction

transformation

mutation

conjugation

TRUE ANSWER : ?
YOUR ANSWER : ?

The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

have the promoter of lacZ or some other reporter gene

allow to monitor the expression of all genes with a promoter similar in sequence to Px

have Px but not the rest of the X coding region

give the same information as from a microarray

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following type of recombination does not require homologous sequences and is utilized by mobile genetic elements that move about chromosomes?

Replicative recombination

Site-specific recombination

Mutagenic recombimation

General recombination

TRUE ANSWER : ?
YOUR ANSWER : ?

Plasmid that carries genes encoding enzymes, which degrade substances such as aromatic compounds, pesticides or sugar are

metabolic plasmid

none of these

F factors

virulence plasmid

TRUE ANSWER : ?
YOUR ANSWER : ?

The chromosomal genes, possessing fertility factor is known as

R factor

F factor

F prime factor

HFr

TRUE ANSWER : ?
YOUR ANSWER : ?

In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by

generalized phage transduction

natural transformation

conjugal transfer by a self-transmissible plasmid

None of these

TRUE ANSWER : ?
YOUR ANSWER : ?

What is the term used for a segment of DNA with one or more genes in the centre and the two ends carrying inverted repeat sequences of nucleotides?

None of these

Plasmid

Transposon

Insertion sequence

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is the cause for drug resistance in tuberculosis?

Transformation

Transduction

Mutation

Conjugation

TRUE ANSWER : ?
YOUR ANSWER : ?

When composite transposons are formed

two IS elements integrate into a chromosome with only a small distance separating them

an IS element integrates with another IS element with the help of a plasmid

a small deletion occurs in the transposase gene of an IS element and plasmid is integrated

a small deletion occurs in the transposase gene of an IS element

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following statement(s) is/are true in regards to F+ x F- mating events?

DNA is transferred from F+ to F- cells

DNA is transferred from F- to F+ cells

No DNA is transferred because F- cells are unable to perform conjugation

No DNA is transferred because F+ cells are unable to perform conjugation

TRUE ANSWER : ?
YOUR ANSWER : ?