MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Which type of plasmid can exist with or without being integrated into the host's chromosome?

Episome

Lisosome

Lysogen

Medisome

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In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by

natural transformation

generalized phage transduction

conjugal transfer by a self-transmissible plasmid

None of these

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Which of the following is the cause for drug resistance in tuberculosis?

Transduction

Mutation

Transformation

Conjugation

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Which of the following type of recombination does not require homologous sequences and is utilized by mobile genetic elements that move about chromosomes?

Replicative recombination

General recombination

Mutagenic recombimation

Site-specific recombination

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The term used for plasmids possessing both RTF and r determinants is

non conjugative plasmids

None of these

conjugative plasmids

non self-transmissible plasmids

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Which of the following is used by microbial genetisists as a tool?

All of these

Bacteriophage

Transposable elements

Plasmids

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Which of the following plamids do not possess information for self transfer to another cell?

Cryptic plasmids

Non-conjugative plasmids

None of these

Conjugative plasmids

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Which of the following term describes the relationship between a virus and host where no new viral particles are produced and the viral genome is replicated along with host chromosome?

Lysis

Conjugation

Transformation

Lysogeny

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The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

transfers antibiotic resistance genes

carries genes encoding the mating apparatus

transfers both strands of the plasmid DNA

usually has a transposon inserted into it

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The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

allow to monitor the expression of all genes with a promoter similar in sequence to Px

have the promoter of lacZ or some other reporter gene

have Px but not the rest of the X coding region

give the same information as from a microarray

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