MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Which type of plasmid can exist with or without being integrated into the host's chromosome?

Medisome

Episome

Lisosome

Lysogen

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In lysogeny,

a bacteriophage genome is integrated into the bacterial genome

DNA-degrading enzymes in the extracellular medium would stop the process

a bacteriophage transfers bacterial DNA

bacteria take up double stranded DNA from the environment

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The transposase gene encodes an enzyme that facilitate

general recombination

site-specific integration of transposable elements

None of these

viral replication within a genome

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Diagnostic DNA probes have been developed for

All of these

Hepatitis B virus

Human immunodeficiency virus

Mycobacterium tuberculosis

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What is the term used for a segment of DNA with one or more genes in the centre and the two ends carrying inverted repeat sequences of nucleotides?

None of these

Plasmid

Insertion sequence

Transposon

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The plasmid which makes the host more pathogenic is

F factors

None of these

Metabolic plasmid

Virulence plasmid

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The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

have Px but not the rest of the X coding region

give the same information as from a microarray

have the promoter of lacZ or some other reporter gene

allow to monitor the expression of all genes with a promoter similar in sequence to Px

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The correct term for the transfer of genetic material between bacteria in direct physical contact is

transduction

replication

conjugation

transformation

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The term used for acquisition of naked DNA from its environment and its incorporation in their genome by a bacterium is

transduction

lysogenic conversion

transformation

conjugation

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Which of the following is the cause for drug resistance in tuberculosis?

Conjugation

Mutation

Transformation

Transduction

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