MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Which type of plasmid can exist with or without being integrated into the host's chromosome?

Medisome

Episome

Lisosome

Lysogen

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The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

have Px but not the rest of the X coding region

allow to monitor the expression of all genes with a promoter similar in sequence to Px

give the same information as from a microarray

have the promoter of lacZ or some other reporter gene

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What is term used for a bacterial cell that is able to take up naked DNA?

Infected

Competent

Liable

Complementary

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The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

usually has a transposon inserted into it

carries genes encoding the mating apparatus

transfers antibiotic resistance genes

transfers both strands of the plasmid DNA

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Which of the following transport bacterial DNA to other bacteria via bacteriophages?

Translation

Conjugation

Transformation

Transduction

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Which of the following type of recombination does not require homologous sequences and is utilized by mobile genetic elements that move about chromosomes?

General recombination

Mutagenic recombimation

Replicative recombination

Site-specific recombination

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Which of the following is used by microbial genetisists as a tool?

All of these

Transposable elements

Bacteriophage

Plasmids

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Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

General recombination

Site-specific recombination

Mutagenic recombimation

Replicative recombination

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Which of the following term describes the relationship between a virus and host where no new viral particles are produced and the viral genome is replicated along with host chromosome?

Lysogeny

Lysis

Transformation

Conjugation

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In the extracellular medium, DNA-degrading enzymes would likely be to prevent transfer of DNA by

generalized phage transduction

natural transformation

conjugal transfer by a self-transmissible plasmid

None of these

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