MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Non-conjugative plasmids

None of these

Cryptic plasmids

Conjugative plasmids

allow to monitor the expression of all genes with a promoter similar in sequence to Px

give the same information as from a microarray

have Px but not the rest of the X coding region

have the promoter of lacZ or some other reporter gene

Transposable elements

Bacteriophage

All of these

Plasmids

transfers antibiotic resistance genes

usually has a transposon inserted into it

transfers both strands of the plasmid DNA

carries genes encoding the mating apparatus

gives direct measurement of mRNA level

carries DNA segments from many different genes

All of these

is not constructed by cloning

a small deletion occurs in the transposase gene of an IS element and plasmid is integrated

two IS elements integrate into a chromosome with only a small distance separating them

a small deletion occurs in the transposase gene of an IS element

an IS element integrates with another IS element with the help of a plasmid

Metabolic plasmid

F factors

Virulence plasmid

None of these

virulence plasmid

metabolic plasmid

F factors

none of these

Mutation

Conjugation

Transformation

Transduction

transduction

conjugation

trasnscription

replication