MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Penicillin resistance in staphylococci is acquired due to

transformation

transduction

mutation

conjugation

TRUE ANSWER : ?
YOUR ANSWER : ?

The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

give the same information as from a microarray

allow to monitor the expression of all genes with a promoter similar in sequence to Px

have Px but not the rest of the X coding region

have the promoter of lacZ or some other reporter gene

TRUE ANSWER : ?
YOUR ANSWER : ?

When composite transposons are formed

an IS element integrates with another IS element with the help of a plasmid

a small deletion occurs in the transposase gene of an IS element and plasmid is integrated

two IS elements integrate into a chromosome with only a small distance separating them

a small deletion occurs in the transposase gene of an IS element

TRUE ANSWER : ?
YOUR ANSWER : ?

The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

transfers both strands of the plasmid DNA

usually has a transposon inserted into it

carries genes encoding the mating apparatus

transfers antibiotic resistance genes

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following statement describes plasmids?

None of these

Small, circular DNA molecules that can exist independently of chromosomes commonly found in bacteria

A complex membrane structure that covers the chromosome of bacteria

Another name for a protoplast

TRUE ANSWER : ?
YOUR ANSWER : ?

The correct term for the transfer of genetic material between bacteria in direct physical contact is

transduction

replication

transformation

conjugation

TRUE ANSWER : ?
YOUR ANSWER : ?

The term used for plasmids possessing both RTF and r determinants is

non conjugative plasmids

non self-transmissible plasmids

conjugative plasmids

None of these

TRUE ANSWER : ?
YOUR ANSWER : ?

The plasmids can be eliminated from a cell by the process known as

breaking

curing

expulsion

fixing

TRUE ANSWER : ?
YOUR ANSWER : ?

The type of recombination that commonly occurs between a pair of homologous DNA sequences is,

general recombination

mutagenic recombination

site-specific recombination

replicative recombination

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following statement(s) is/are true in regards to F+ x F- mating events?

No DNA is transferred because F+ cells are unable to perform conjugation

DNA is transferred from F+ to F- cells

No DNA is transferred because F- cells are unable to perform conjugation

DNA is transferred from F- to F+ cells

TRUE ANSWER : ?
YOUR ANSWER : ?