MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

have Px but not the rest of the X coding region

allow to monitor the expression of all genes with a promoter similar in sequence to Px

have the promoter of lacZ or some other reporter gene

give the same information as from a microarray

TRUE ANSWER : ?
YOUR ANSWER : ?

R factors involved in plant-microbe interactions are

bacterial proteins

essential for transfer of DNA to plant cells

also called opines

plant proteins

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

Site-specific recombination

Mutagenic recombimation

Replicative recombination

General recombination

TRUE ANSWER : ?
YOUR ANSWER : ?

Diagnostic DNA probes have been developed for

Human immunodeficiency virus

Mycobacterium tuberculosis

All of these

Hepatitis B virus

TRUE ANSWER : ?
YOUR ANSWER : ?

The term used for acquisition of naked DNA from its environment and its incorporation in their genome by a bacterium is

transformation

transduction

conjugation

lysogenic conversion

TRUE ANSWER : ?
YOUR ANSWER : ?

Who discovered transposons (jumping genes)?

Griffith

Harvey

Abelson

McClintock

TRUE ANSWER : ?
YOUR ANSWER : ?

A microarray differs from a gene fusion in that, it

carries DNA segments from many different genes

All of these

gives direct measurement of mRNA level

is not constructed by cloning

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is used by microbial genetisists as a tool?

Transposable elements

All of these

Bacteriophage

Plasmids

TRUE ANSWER : ?
YOUR ANSWER : ?

Which type of plasmid can exist with or without being integrated into the host's chromosome?

Episome

Medisome

Lysogen

Lisosome

TRUE ANSWER : ?
YOUR ANSWER : ?

What is term used for a bacterial cell that is able to take up naked DNA?

Liable

Infected

Competent

Complementary

TRUE ANSWER : ?
YOUR ANSWER : ?

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