MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

Which of the following statement(s) is/are true in regards to F+ x F- mating events?

No DNA is transferred because F- cells are unable to perform conjugation

DNA is transferred from F- to F+ cells

No DNA is transferred because F+ cells are unable to perform conjugation

DNA is transferred from F+ to F- cells

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YOUR ANSWER : ?

What is the term used for a segment of DNA with one or more genes in the centre and the two ends carrying inverted repeat sequences of nucleotides?

None of these

Transposon

Plasmid

Insertion sequence

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Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

Site-specific recombination

General recombination

Replicative recombination

Mutagenic recombimation

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The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

allow to monitor the expression of all genes with a promoter similar in sequence to Px

give the same information as from a microarray

have Px but not the rest of the X coding region

have the promoter of lacZ or some other reporter gene

TRUE ANSWER : ?
YOUR ANSWER : ?

The plasmid which makes the host more pathogenic is

None of these

Metabolic plasmid

Virulence plasmid

F factors

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The plasmid-mediated properties is/are

resistance to antibiotics

production of enterotoxin

fermentation of lactose

all of these

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Which type of plasmid can exist with or without being integrated into the host's chromosome?

Medisome

Lisosome

Episome

Lysogen

TRUE ANSWER : ?
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R factors involved in plant-microbe interactions are

plant proteins

essential for transfer of DNA to plant cells

bacterial proteins

also called opines

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The transducing particles carry only specific portions of the bacterial genome in which of the following transduction?

None of these

General transduction

Abortive transduction

Specialized transduction

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What information can be generated by interrupted mating experiments?

Proteomics of the bacteria

Levels of DNA homology

DNA nucleotide sequences

Bacterial genome maps

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