MICROBIAL RECOMBINATION AND GENE TRANSFER QUIZ

What information can be generated by interrupted mating experiments?

DNA nucleotide sequences

Levels of DNA homology

Bacterial genome maps

Proteomics of the bacteria

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Which of the following statement(s) is/are true in regards to F+ x F- mating events?

No DNA is transferred because F+ cells are unable to perform conjugation

DNA is transferred from F- to F+ cells

No DNA is transferred because F- cells are unable to perform conjugation

DNA is transferred from F+ to F- cells

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The transposase gene encodes an enzyme that facilitate

site-specific integration of transposable elements

general recombination

viral replication within a genome

None of these

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F factor plasmids play a major role in

trasnscription

transduction

conjugation

replication

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The plasmids can be eliminated from a cell by the process known as

curing

expulsion

fixing

breaking

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A microarray differs from a gene fusion in that, it

All of these

is not constructed by cloning

carries DNA segments from many different genes

gives direct measurement of mRNA level

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The term used for plasmids possessing both RTF and r determinants is

conjugative plasmids

None of these

non conjugative plasmids

non self-transmissible plasmids

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Inverted repeat sequences at each end and a gene encoding transposase is contained in which of the following transposable element?

Composite transposon

Virus

Plasmid

Insertion element

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Which of the following type of recombination does not require homologous sequences and is important for the integration of viral genomes into bacterial chromosomes?

Mutagenic recombimation

Site-specific recombination

General recombination

Replicative recombination

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In lysogeny,

a bacteriophage transfers bacterial DNA

a bacteriophage genome is integrated into the bacterial genome

DNA-degrading enzymes in the extracellular medium would stop the process

bacteria take up double stranded DNA from the environment

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