MEDIA REQUIRED FOR PLANT CELL CULTURE QUIZ

it is removed aseptically from the culture and is autoclaved before use

It contains uncharacterized growth factor released by growing cells

It is liquid removed from the suspension of fast growing cells

It is used in the culture of regenerating protoplast

40-50 g/l

20-30 g/l

10-15 g/l

60-70 g/l

Activated charcoal

Citric acid

Silver thiosulphate

Succinic acid

Sucrose

Fructose

Maltose

Glucose

both (a) and (b)

ammonium salts are used

synthetic buffers such as Tris, MES or HEPS are used

organic acid such as citric, fumaric, malic and succinic acid is used

in specialized secondary metabolite production

to adjust the osmotic potential of the media in short term treatment for regeneration

Both (a) and (b)

none of these

utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise

utilization of both ammonium and nitrate result in drop in pH

utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise

utilization of both ammonium and nitrate result in rise in pH

Auxins

Cytokinins

Brassinosteroids

Gibberellins

Auxins

Gibberellins

Brassinosteroids

Cytokinins

Conjugated to inactive form by plant cells

Gets easily oxidized

All of these

It is unstable in solution