INDUS VALLEY CIVILISATION QUIZ

An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

there was one antibody and one antigen

the antibody was impure

the temperature was too high

there was more than one antigen

TRUE ANSWER : ?
YOUR ANSWER : ?

Immunoaffinity chromatography can be used in biochemical applications to

break down antigen and analyze quantitatively

None of these

break down antibody structure

purify protein antigen

TRUE ANSWER : ?
YOUR ANSWER : ?

RAST test (radio allergosorbent test) is often used to detect

antigen associated with allergies (IgE)

None of these

antibodies associated with allergies (IgE)

bacteriophages

TRUE ANSWER : ?
YOUR ANSWER : ?

Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight?

Open circular, linear plasmid, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

Linear plasmid, open circular, super coiled plasmid

Open circular, super coiled, linear plasmid DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

What radioactive molecule is used to end-label an oligonucleotide?

Any of these

(gamma)-32P-ATP

(alpha)-32P-ATP

(alpha)-32P-dUTP

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following radioactive element is generally involved in RIA?

Iodine-125

Carbon-14

All of these

Tritium

TRUE ANSWER : ?
YOUR ANSWER : ?

Rocket Immunodiffusion is also known as

double-diffusion

electroimmunodiffusion

gel diffusion

none of these

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following is a positively charged matrix for ion exchange chromatography?

None of these

CM cellulose

DEAE cellulose

Phosphocellulose

TRUE ANSWER : ?
YOUR ANSWER : ?

In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen parallel to each other

the antibody will migrate towards cathode

electrophoresis will drive the antibody and antigen toward each other

the antibody will migrate towards anode

TRUE ANSWER : ?
YOUR ANSWER : ?

What would happen if serum is omitted from the ELISA, but all other steps remain same?

Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

Both (a) and (b)

Anti-human Ig-conjugate would not bind and be washed away

The OD values would be nearly the same as the assay control

TRUE ANSWER : ?
YOUR ANSWER : ?